Chinese Journal OF Rice Science >
Identification and Fine Mapping of a Grain Width Mutant gw4 in Rice
#These authors contributed equally to this work;
Received date: 2020-06-10
Revised date: 2020-08-06
Online published: 2021-05-10
【Objective】Grain size is an important agronomic trait that determines yield and appearance quality in rice. Screening and identification of new grain size mutants will lay a foundation for further study of the regulation mechanism of grain development. 【Method】A grain width mutant gw4（grain width on chromosome 4）was obtained from 1% EMS mutagenized population of Zhonghua 11. The scanning electron microscope analysis of the outer and inner surfaces cells of glumes was carried out, and grain size and other agronomic traits were measured at the heading and maturity stages, respectively. With the map-based cloning, the mutant individuals derived from F2 population of gw4 and TN1 were used for fine mapping. The candidate gene was determined by gene sequencing based on bioinformatics analysis, and expression analysis of grain size-related genes was carried out by the RT-PCR. 【Result】Besides narrow grains, gw4 also showed decreased phenotype values in plant height, grain length, thousand grain weight, grain number, number of primary and secondary rachis branches. Scanning electron microscope observation presented that both cell length and width of inner and outer surfaces cells of glumes were smaller than the wild type. Genetic analysis showed that the narrow grain phenotype was regulated by a single recessive nuclear gene, which was fine mapped to a region of 31.74 kb between the two markers BS6 and EX49 on chromosome 4. Sequence analyses revealed a single base substitution of G to A was found in LOC_Os04g01590 which resulted in a substitution of glycine to aspartic acid, and RT-PCR analysis indicated that it was mainly expressed in young panicles with declined expression level in mutant as compared with the wild type. 【Conclusion】GW4 is mainly involved in grain width development, and its candidate gene should be LOC_Os04g01590, which lays a foundation for further perfecting the genetic regulatory network of grain size.
Key words： rice; grain size; grain width; gene mapping; appearance quality
Xuemei DENG, Peng HU, Yueying WANG, Yi WEN, Yiqing TAN, Hao WU, Kaixiong WU, Junge WANG, Linlin HOU, Lixin ZHU, Li ZHU, Guang CHEN, Dali ZENG, Guangheng ZHANG, Longbiao GUO, Zhenyu GAO, Deyong REN, Qian QIAN, Jiang HU . Identification and Fine Mapping of a Grain Width Mutant gw4 in Rice[J]. Chinese Journal OF Rice Science, 2021 , 35(3) : 259 -268 . DOI: 10.16819/j.1001-7216.2021.01204
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