Chinese Journal OF Rice Science >
Location and Candidate Gene Analysis of Rice Clustered Spikelets Gene OsCL6
Received date: 2020-06-10
Revised date: 2020-08-06
Online published: 2021-05-10
【Objective】The mapping and candidate gene analysis of rice clustered spikelet genes help clone the functional genes for clustered spikelet caused by shortening of the branch length in the process of panicle development. 【Method】A stable clustered spikelet mutant cl6 was obtained through the space mutation breeding technique with Yuenongsimiao, a good quality rice cultivar in Guangdong Province as material. F1 and F2 mapping populations were constructed by crossing cl6 with Yuejinyinzhan. The clustered spikelet gene was mapped and candidate genes were predicted and evaluated by combining with transcriptome sequencing. 【Result】Genetic analysis showed that the trait was controlled by a pair of semi-dominant gene. The gene was mapped to an interval about 416 kb on chromosome 6 by Bulked Segregant Analysis(BSA). Furthermore, OsFBK16 was selected as the best candidate gene through expression profiling analysis, RNA-seq, gene sequence differences analysis and qRT-PCR verification. A 9 bp insertion mutation in the 5'-UTR region of this gene suggests that it may regulate transcription or translation process through secondary structural changes and participating in the differentiation of stems during the development of young panicle of rice. 【Conclusion】It will lay a theoretical foundation for analyzing the shortening mechanism of secondary branches and spikelet peduncle length.
Wei LIU, Zhanhua LU, Dongbai LU, Xiaofei WANG, Shiguang WANG, Jia XUE, Xiuying HE . Location and Candidate Gene Analysis of Rice Clustered Spikelets Gene OsCL6[J]. Chinese Journal OF Rice Science, 2021 , 35(3) : 238 -248 . DOI: 10.16819/j.1001-7216.2021.0604
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