Chinese Journal OF Rice Science >
Identification of a Knockout Mutant of OsWOX3B Gene in Rice (Oryza sativa L.)
Received date: 2020-06-02
Revised date: 2020-09-04
Online published: 2021-12-07
【Objective】The OsWOX3B gene regulates leaf morphology and trichome development in rice. This study aims to further understand the role of OsWOX3B gene in regulating rice development. 【Method】In this study, OsWOX3B of indica rice variety R401 was knocked out by CRISPR/Cas9 gene editing technique. The mutation sites and phenotype of the obtained materials were analyzed as well as the expression levels of related genes. 【Result】The 341st base of the coding region of OsWOX3B of the obtained plant was changed from T to C, and the 395th-397th bases were absent, and the leaves and glumes of the mutant were glabrous, the same as the phenotype of dep/nuda/glr1. Therefore, the plant was Oswox3b mutant. In addition to the reported loss-of-function phenotype of OsWOX3B, new phenotypes of Oswox3b were found in this study. Compared with wild-type R401, mutant Oswox3b showed prolonged growth duration, decreased tiller number, wider and shorter leaves, longer panicle and more grains per panicle. At the same time, the mutant Oswox3b had increased number of vascular bundles in the blade, increased distance between the small vascular bundles. The changes in the expression levels of the two genes related to the development of lateral organs in the mutant Oswox3b were also consistent with the phenotypic changes. 【Conclusion】A new mutant of rice OsWOX3B gene was identified that affects lateral organ development in rice.
Key words： rice (Oryza sativa L.); lateral organs; vascular bundle; growth duration
Yali ZHENG, Linchuang YU, Xiaoxiao AN, Xinle CHENG, Lijun REN, Zilong SU, Xiaoya ZHENG, Tao LAN . Identification of a Knockout Mutant of OsWOX3B Gene in Rice (Oryza sativa L.)[J]. Chinese Journal OF Rice Science, 2021 , 35(2) : 112 -120 . DOI: 10.16819/j.1001-7216.2021.0602
| [1] | 矫永庆. 水稻理想株型基因Ideal Plant Architecture 1(IPA1)的克隆与功能研究[D]. 北京: 中国科学院, 2010. |
| [1] | Jiao Y Q.Cloning and functional study of ideal plant architecture 1(IPA1)[D]. Beijing: Chinese Academy of Sciences, 2010. (in Chinese with English abstract). |
| [2] | 于燕杰, 张大兵, 袁政. WOX蛋白家族调控干细胞发育分子机制的研究进展[J]. 植物学报, 2016, 51(4): 565-574. |
| [2] | Yu Y J, Zhang D B, Yuan Z.Progress in the molecular mechanism of WOX protein family regulating stem cell development[J]. Chinese Bulletin of Botany, 2016, 51(4): 565-574. (in Chinese with English abstract) |
| [3] | 高丽, 孙祎敏, 邵铁梅, 孔卫娜, 崔润丽, 卢楠, 仵陶. 植物WUSCHEL-related homeobox (WOX) 家族研究进展[J]. 生物技术通报, 2015, 31(5): 7-12. |
| [3] | Gao L, Sun W M, Shao T M, Kong W N, Cui R L, Lu N, Wu T.Research progress of WUSCHEL related homeobox (WOX) family of plants[J]. Biotechnology Bulletin, 2015, 31(5): 7-12. (in Chinese with English abstract) |
| [4] | Zhao Y, Hu Y, Dai M, Huang L, Zhou D X.The WUSCHEL-related homeobox gene WOX11 is required to activate shoot-borne crown root development in rice[J]. Plant Cell, 2009, 21(3): 736-748. |
| [5] | Zhao Y, Cheng S, Song Y, Huang Y, Zhou S, Liu X, Zhou D X.The interaction between rice ERF3 and WOX11 promotes crown root development by regulating gene expression involved in cytokinin signaling[J]. Plant Cell, 2015, 27(9): 2469-2483. |
| [6] | Chen G, Feng H, Hu Q, Qu H, Chen A, Yu L, Xu G.Improving rice tolerance to potassium deficiency by enhancing OsHAK16p: WOX11-controlled root development[J]. Plant Biotechnology Journal, 2015, 13(6): 833-848. |
| [7] | Kamiya N, Nagasaki H, Morikami A, Sato Y, Matsuoka M.Isolation and characterization of a rice WUSCHEL-type homeobox gene that is specifically expressed in the central cells of a quiescent center in the root apical meristem[J]. Plant Journal, 2003, 35(4): 429-441. |
| [8] | Chu H W, Liang W Q, Li J, Hong F, Wu Y F, Wang L K, Wang J, Wu P, Liu C M, Zhang Q F, Xu J, Zhang D B.A CLE-WOX signaling module regulates root meristem maintenance and vascular tissue development in rice[J]. Journal of Experimental Botany, 2013, 64: 5359-5369. |
| [9] | Wang W F, Li G, Zhao J, Chu H W, Lin W H, Zhang D B, Wang Z Y, Liang W Q.DWARF TILLER1, a WUSCHEL-related homeobox transcription factor, is required for tiller growth in rice[J/OL]. PLoS Genetics, 2014, 10: e1004154. |
| [10] | Lu Z F, Shao G N, Xiong J S, Jiao Y Q, Wang J, Liu G F, Meng X B, Liang Y, Xiong G S, Wang Y H, Li J Y.MONOCULM 3, an ortholog of WUSCHEL in rice, is required for tiller bud formation[J]. Journal of Genetics and Genomics, 2015, 42(2): 71-78. |
| [11] | Yasui Y, Ohmori Y, Takebayashi Y, Sakakibara H, Hirano H Y.WUSCHEL-RELATED HOMEOBOX4 acts as a key regulator in early leaf development in rice[J/OL].PLoS Genetics, 2018, 14: e1007365. |
| [12] | Cho S H, Kang K, Lee S H, Lee I J, Paek N C.OsWOX3A is involved in negative feedback regulation of the gibberellic acid biosynthetic pathway in rice (Oryza sativa)[J]. Journal of Experimental Botany, 2016, 67(6): 1677-1687. |
| [13] | Cho S H, Yoo S C, Zhang H, Pandeya D, Koh H J, Hwang J Y, Kim G T, Paek N C.The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL-related homeobox 3A (OsWOX3A) and function in leaf, spikelet, tiller and lateral root development[J]. New Phytologist, 2013, 198(4): 1071-1084. |
| [14] | Dai M, Hu Y, Zhao Y, Liu H, Zhou D X.A WUSCHEL-LIKE HOMEOBOX gene represses a YABBY gene expression required for rice leaf development[J]. Plant Physiology, 2007, 144(1): 380-390. |
| [15] | Obara M, Ikeda K, Itoh J I, Nagato Y.Characterization of leaf lateral symmetry 1 mutant[J]. Breeding Science, 2004, 54(2): 157-163. |
| [16] | Honda E, Yew C L, Yoshikawa T, Sato Y, Hibara K I, Itoh J I.LEAF LATERAL SYMMETRY1, a member of the WUSCHEL-RELATED HOMEOBOX3 gene family, regulates lateral organ development differentially from other paralogs, NARROW LEAF2 and NARROW LEAF3 in rice[J]. Plant Cell Physiology, 2018, 59(2): 376-391. |
| [17] | Angeles-Shim R B, Asano K, Takashi T, Shim J, Kuroha T, Ayano M, Ashikari M. A WUSCHEL-related homeobox 3B gene, depilous (dep), confers glabrousness of rice leaves and glumes[J]. Rice, 2012, 5(1): 28. |
| [18] | Zhang H L, Wu K, Wang Y F, Peng Y, Hu F Y, Wen L, Han B, Qian Q, Teng S.A WUSCHEL-like homeobox gene, OsWOX3B responses to NUDA/GL-1 locus in rice[J]. Rice, 2012, 5(1): 30. |
| [19] | Li J, Yuan Y, Lu Z, Yang L, Gao R, Lu J, Li J, Xiong G.Glabrous rice 1, encoding a homeodomain protein, regulates trichome development in rice[J]. Rice, 2012, 5(1): 32. |
| [20] | Sun W Q, Gao D W, Xiong Y, Tang X X, Xiao X F, Wang C G, Yu S B.Hairy Leaf 6, an AP2/ERF transcription factor, interacts with OsWOX3B and regulates trichome formation in rice[J]. Molecular Plant, 2017, 10(11): 1417-1433. |
| [21] | Nishimura A, Ito M, Kamiya N, Sato Y, Matsuoka M.OsPNH1 regulates leaf development and maintenance of the shoot apical meristem in rice[J]. Plant Journal, 2002, 30(2): 189-201. |
| [22] | Tanaka W, Toriba T, Ohmori Y, Yoshida A, Kawai A, Mayama-Tsuchida T, Ichikawa H, Mitsuda N, Ohme-Takagi M, Hirano H Y.The YABBY gene TONGARI-BOUSHI1 is involved in lateral organ development and maintenance of meristem organization in the rice spikelet[J]. Plant Cell, 2012, 24(1): 80-95. |
| [23] | Ishiwata A, Ozawa M, Nagasaki H, Kato M, Noda Y, Yamaguchi T, Nosaka M, Shimizu-Sato S, Nagasaki A, Maekawa M, Hirano H Y, Sato Y.Two WUSCHEL-related homeobox genes, narrow leaf 2 and narrow leaf 3, control leaf width in rice[J]. Plant and Cell Physiology, 2013, 54(5): 779-792. |
| [24] | Chen M, Luo J, Shao G, Wei X, Tang S, Sheng Z, Song J, Hu P.Fine mapping of a major QTL for flag leaf width in rice, qFLW4, which might be caused by alternative splicing of NAL1[J]. Plant Cell Reports, 2012, 31(5): 863-872. |
| [25] | Qi J, Qian Q, Bu Q, Li S, Chen Q, Sun J, Liang W, Zhou Y, Chu C, Li X, Ren F, Palme K, Zhao B, Chen J, Chen M, Li C.Mutation of the rice narrow leaf1 gene, which encodes a novel protein, affects vein patterning and polar auxin transport[J]. Plant Physiology, 2008, 147(4): 1947-1959. |
| [26] | Zhang X, Zong J, Liu J L, Yin J Y, Zhang D B.Genome-wide analysis of WOX gene family in rice, sorghum, maize, Arabidopsis and poplar[J]. Journal of Integrative Plant Biology, 2010, 52(11): 1016-1026. |
| [27] | Yoo S C, Cho S H, Paek N C. Rice WUSCHEL-related homeobox 3A (OsWOX3A) modulates auxin-transport gene expression in lateral root and root hair development[J/OL]. Plant Signaling and Behavior, 2013, 8(10): 10, e25929. |
/
| 〈 |
|
〉 |