Chinese Journal OF Rice Science >
CRISPR/Cas9-mediatedEditing of AFP1Improves Rice Stress Tolerance
Received date: 2020-05-03
Revised date: 2020-05-27
Online published: 2021-01-10
【Objective】It is important to reveal the functions of rice AFP1 in response to abiotic stress, and createnewstress-resistant rice germplasm in innovative ways.【Method】Excellent indica restoring line Huazhan was used as transgenic receptor material to generate afp1 mutants usingCRISPR/Cas9 system, and abiotic stress tolerances of afp1 knockout lines were preliminaryanalyzed. 【Results】The editing efficiency of AFP1 in two targets were 66.67% and 75.00%, respectively.In all mutant plants, only insertions and deletions occurred.About 90% of mutant bases were small fragments,with a lengthless than 5bp.Six homozygous mutants without genetically modified ingredients were generated.Under normal conditions, plant height and seed setting rate of afp1 mutants were reduced, but effective tiller number per plant and panicle length were significantly increased. Interestingly, the yield per plant of mutants changed between-4.06%and +11.75%. Compared with the wild type, the ABA sensitivity and water loss rate of afp1 mutants were obviously decreased. Drought, heat and osmotic stress resistances were significantly improved.【Conclusion】MutationofAFP1genecouldimprovemultiple abiotic stress resistances in rice.
Key words： rice; CRISPR/Cas9 gene editing technology; AFP1; abiotic stresstolerance
Tianshun ZHOU, Dong YU, Ling LIU, Ning OUYANG, Guilong YUAN, Meijuan DUAN, Dingyang YUAN . CRISPR/Cas9-mediatedEditing of AFP1Improves Rice Stress Tolerance[J]. Chinese Journal OF Rice Science, 2021 , 35(1) : 11 -18 . DOI: 10.16819/j.1001-7216.2021.0503
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