Chinese Journal OF Rice Science >
Diversity of Midgut Microbial Community of ChilosuppressalisExposed to Chlorobenzamide
Received date: 2020-01-06
Revised date: 2020-04-20
Online published: 2020-11-10
【Objective】We aim toilluminatethestructural changes in the midgut microbial community of Chilosuppressalis exposed to chlorobenzamide.【Method】Macrogenomic sequencing and in vitro culturewere used to analyze the diversity of midgut microbiotaof Chilosuppressalistreatedwith different concentrations (100, 200, 400µg/mL) of chlorobenzamide.【Result】The results showed that chlorobenzamide treatmentdecreased the abundance anddiversityof the midgut bacteria of Chilosuppressalis,and the OTU number and specific OTU number of the intestinal bacteria of treated population were lower than those of the control;the proportion of Morganella, Provincia and Proteus in the chlorobenzamide treated population increased significantly. Meanwhile, the result of in vitro culture indicated besides enterobacter,Lelliottinimipressuralis, Leclerciadecarboxylata, Pantoeaagglomeranswere isolated from chlorobenzamide treated Chilosuppressalis population. COG classification and KEGG analysis didn’t show significant difference in number and relative abundance of functional genes among control and three chlorobenzamide treated populations.【Conclusion】The study had clarified the diversity of intestinal bacteria and the relationshipbetween gutbacteria of Chilosuppressalis andthe formation of chlorobenzamide resistance, and will lay a scientific basis for the role of intestinal bacteria in resistance of Chilosuppressalistochlorobenzamide.
Juefeng ZHANG, Qin ZHANG, Fang LI, Haiying ZHONG, Jianming CHEN . Diversity of Midgut Microbial Community of ChilosuppressalisExposed to Chlorobenzamide[J]. Chinese Journal OF Rice Science, 2020 , 34(6) : 586 -594 . DOI: 10.16819/j.1001-7216.2020.0101
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