Chinese Journal OF Rice Science >
Identification of Blast Disease Resistance-related Genes by Genomic Sequence Comparison of Rice Variety Ziyu 44 and Jiangnanxiangnuo
About author:#These authors contributed equally to this work
Received date: 2019-06-12
Revised date: 2019-06-28
Online published: 2020-01-10
【Objective】 Ziyu 44 is indigenous japonica rice from Yunnan Province of China with durable broad-spectrum resistance to M. oryzae. In order to identify novel candidate rice blast resistance-related genes from Ziyu 44, 【Method】 we performed the whole genome sequencing of Ziyu 44 and susceptible variety Jiangnanxiangnuo(JNXN). Then we checked and summarized SNPs/InDels of high-throughput sequencing data by software GATK(3.4-46), and screened out disease resistance-related genes with SNPs/InDels polymorphic loci at DNA level of Ziyu 44 and JNXN.【Result】4 118 170 045 bp and 2 995 054 509 bp genomic data of Ziyu 44 and Jiangnanxiangnuo were respectively produced using Hiseq X10 PE150 platform. The alignment rates to the reference genomes (Ensembl release 31) were 98.56% and 98.30%, respectively. A total of 922 resistance-related differential genes were identified between Ziyu 44 and JNXN. Further, combined with the result of gene mapping, we identified a new blast resistance candidate gene in Ziyu 44. 【Conclusion】Our results provide valuable information for cloning of new rice blast resistance new genes, and lay an important foundation for exploring the molecular mechanism of durable broad-spectrum resistance to rice blast in Ziyu 44.
Key words： rice; rice blast; genome sequencing; resistance-related genes
Jinlu LI, Hui ZHANG, Zeyu JIAO, Jianyu LIU, Guangyu HAN, Xiaoxuan ZHUO, Qiong LUO . Identification of Blast Disease Resistance-related Genes by Genomic Sequence Comparison of Rice Variety Ziyu 44 and Jiangnanxiangnuo[J]. Chinese Journal OF Rice Science, 2020 , 34(1) : 8 -16 . DOI: 10.16819/j.1001-7216.2020.9066
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