Chinese Journal OF Rice Science >
Thermo-sensitive Male Sterile Line Created by Editing TMS5 Gene in japonica Rice
Received date: 2019-03-28
Revised date: 2019-04-23
Online published: 2019-09-10
【Objective】The breeding of thermo-sensitive male sterile rice lines is a key step in the breeding of two-line hybrid rice. TMS5 is the key gene that modulates pollen fertility of rice at different temperatures and has been widely used in rice breeding. In order to accelerate the breeding process of thermo-sensitive sterility lines, 【Method】CRISPR/Cas9 technology was used to edit TMS5 with a japonica rice variety Wuyunjing 7 as a material. 【Results】Sequencing analysis showed that six homozygous mutants screened from 22 strains of T0 generation produced the same genetic variation, and one base “A” was inserted between 44 bp and 45 bp of the second exon of TMS5 of all the six mutants, resulting in pre-maturation of translation. Cytological observation and artificial pollination confirmed that the pollen of tms5 mutant was aborted and the development of female gametes was normal. 【Conclusion】These results indicated that the thermo-sensitive male sterile lines could be obtained rapidly by knocking out the thermo-sensitive male sterile gene TMS5 in japonica rice.
Key words： japonica rice; CRISPR/Cas9; thermo-sensitive male sterile line
Xi DU, Yunyan FEI, Fangquan WANG, Yang XU, Jun WANG, Wenqi LI, Ling ZHAO, Zhihui CHEN, Guohua LIANG, Yong ZHOU, Jie YANG . Thermo-sensitive Male Sterile Line Created by Editing TMS5 Gene in japonica Rice[J]. Chinese Journal OF Rice Science, 2019 , 33(5) : 429 -435 . DOI: 10.16819/j.1001-7216.2019.9035
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