Chinese Journal OF Rice Science >
Analysis of Rice Blast Resistance Genes in japonica Rice Varieties in Liaoning Province
Received date: 2018-06-10
Revised date: 2018-10-18
Online published: 2019-05-10
【Objective】To clarify the rice blast resistance genes in 260 japonica rice varieties in Liaoning Province, evaluate their utilization value, and provide reference for rice breeding,【Method】specific primers of nine rice blast resistance genes were selected to analyze the species, the quantity and the geographical distribution of resistance genes of 260 japonica rice varieties in Liaoning, and the cluster analysis of varieties was based on the detection bands of disease resistance genes. The disease resistance of the nine resistant mongenic rice lines was evaluated by seedling-spray inoculation, natural disease investigation during the active tillering stage and ripening stage.【Result】The number of blast resistance genes in japonica rice from Liaoning varied from 0 to 7, and the frequency of Pikh gene ranked highest, followed by the Pikm and Pi1. Pi9 gene was not detected in all tested varieties, and Pi40 gene was only detected in Liaojing 421 bred by the Liaoning Academy of Agricultural Sciences. There were 62 gene combinations in the rice varieties tested, and based on the clustering analysis of the results of the resistance gene detection, 260 japonica rice materials can be divided into 11 groups. Among the nine resistant mongenic lines, Pi40 showed the best resistance, followed by Pi9, Pigm and Pita.【Conclusion】The resistance genes Pi9, Pi40, Pita, and Pigm have important application potential in breeding in Liaoning Province. 80.38% of the tested varieties concentrate in groups V, VI, VII and IX. There is no obvious geographical difference; the types of disease resistance gene combinations of the tested varieties are relatively less abundant and the genetic relationship is relatively close.
Key words： japonica rice; rice blast; resistance gene
Zhengyan PAN, Fulin QIU, Guilan LÜ, Xiufang MA, Wenqi SHANG, Yuanjun BAI, Zhengjin XU . Analysis of Rice Blast Resistance Genes in japonica Rice Varieties in Liaoning Province[J]. Chinese Journal OF Rice Science, 2019 , 33(3) : 241 -248 . DOI: 10.16819/j.1001-7216.2019.8071
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