Chinese Journal OF Rice Science >
Identification and Fine Mapping of a Narrow Leaf Mutant nal12 in Rice
#These authors contributed equally to this work;
Received date: 2018-11-20
Revised date: 2018-12-18
Online published: 2019-05-10
【Objective】Leaf is a vital component of ideal plant type in rice. Screening and identification of new leaf mutants can lay the foundation for studying leaf development and constructing ideal leaf morphology. 【Method】A novel rice mutant named narrow leaf 12 (nal12) was identified from an EMS mutagenized population of Wuyunjing 31. The leaf length, leaf width, numbers of large and small veins from flag leaf to the 4th leaf from the top were measured, and the histological section and microscopic observation were conducted at the maturity stage. The F2 segregating population was constructed from a cross between nal12 and indica variety TN1, and 1709 narrow-leaf individuals were selected for fine mapping of NAL12 with existing SSR, STS and developed molecular markers. 【Result】The nal12 showed narrow leaves at seedling stage, and exhibited dwarf, increased tillers and thinner stems at maturity stage. According to the anatomy observation of tissue sections, the narrow leaf phenotype of nal12 was caused by the decrease in the number of large veins and small veins compared with the wild type. Genetic analysis indicated that the narrow leaf trait was regulated by a single recessive nuclear gene, and NAL12 was finally mapped to a range of 64.7 kb between LC2-RF37 and LC4R-RF39 markers on chromosome 10. A total of 10 ORFs (open reading frames) were found and there were no narrow-leaf related genes reported in this region. qRT-PCR analysis revealed that the mutation of NAL12 was involved in the expression level of auxin synthesis and transport-related genes. 【Conclusion】NAL12 is a new leaf-shape regulatory gene, which lay a foundation for further research on rice leaf development and enrich its molecular regulatory network.
Key words： rice; narrow leaf; NAL12; gene mapping
Yi WEN, Yunxia FANG, Peng HU, Yueying WANG, Linlin HOU, Yiqing TAN, Lixin ZHU, Xuemei DENG, Dali ZENG, Guangheng ZHANG, Longbiao GUO, Li ZHU, Guang CHEN, Deyong REN, Yuchun RAO, Dawei XUE, Qian QIAN, Jiang HU . Identification and Fine Mapping of a Narrow Leaf Mutant nal12 in Rice[J]. Chinese Journal OF Rice Science, 2019 , 33(3) : 219 -226 . DOI: 10.16819/j.1001-7216.2019.8125
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