Chinese Journal OF Rice Science >
Identification of QTL for Grain Weight and Grain Shape Using Populations Derived from Residual Heterozygous Lines of indica Rice
Received date: 2018-11-06
Revised date: 2018-12-14
Online published: 2019-03-10
【Objective】Grain weight and grain shape are important factors influencing grain yield and quality in rice. There are quantitative traits controlled by a large number of quantitative trait loci (QTL) that vary greatly in effect, but not much attention has been paid to minor QTL. This study was conducted to detect minor QTL for grain weight and shape in the absence of major-QTL segregation. 【Method】 Following results of a previous QTL mapping study, a residual heterozygous plant was identified from the original population. The new population derived was homozygous in regions where major QTL for grain weight and shape were detected in the previous study, but segregated in 13 segments of other genomic regions. The population was planted in Hangzhou, Zhejiang and Lingshui, Hainan. Thousand-grain weight, grain length and grain width were tested and used for QTL analysis using Windows QTL Cartographer 2.5. 【Result】A total of 22 QTL were detected, distributing on 12 intervals of 10 chromosomes. Ten of them were significant in both locations, and the other two were detected in Hangzhou only. One plant that was only heterozygous in four of the 12 QTL regions was identified, from which one new population was constructed and tested. The QTL effects of the four regions were well validated. 【Conclusion】Our results indicate that the power of detecting minor QTL could be efficiently increased by eliminating the influence of major-QTL segregation. It is also suggested that minor QTL could have a stable effect though it is believed that they are generally subjected to large influence of the genetic background and environmental conditions. Our results also lay a foundation for fine mapping, cloning and marker-assisted selection of minor QTL for grain weight and grain shape in rice.
Andong ZHU, Zhichao SUN, Yujun ZHU, Hui ZHANG, Xiaojun NIU, Yeyang FAN, Zhenhua ZHANG, Jieyun ZHUANG . Identification of QTL for Grain Weight and Grain Shape Using Populations Derived from Residual Heterozygous Lines of indica Rice[J]. Chinese Journal OF Rice Science, 2019 , 33(2) : 144 -151 . DOI: 10.16819/j.1001-7216.2019.8120
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