Chinese Journal OF Rice Science >
Origin, Distribution and Sequence Diversity of Rice Blast Resistance Locus LABR_64 in Rice
Received date: 2018-02-08
Revised date: 2018-06-27
Online published: 2019-01-10
【Objective】 A better understanding of the origin and distribution of disease resistance genes in rice germplasm is useful for breeding highly resistant varieties.【Method】We analyzed sequence diversity of the rice blast resistance locus LABR_64, which contains two homologous genes, LABR_64-1 and LABR_64-2, is located in the allelic region of Pi3/Pi5/Pii/Pi15 on rice chromosome 9. In addition, we analyzed the microsynteny of the LABR_64 orthologous region in different monocotyledons.【Result】The presence frequency (PF) of LABR_64 is 16%. All of the japonica rice cultivars carrying LABR_64 are highly resistant to rice blast and all of those without the locus are susceptible. In addition, the PF of LABR_64 in the indica subpopulation is lower than 5%. Although LABR_64 is correlated with the resistance to rice blast, many indica rice cultivars without LABR_64 are also resistant to rice blast. We also found that the LABR_64-2 sequence is much conserved. Moreover, the microsynteny analysis of the LABR_64 orthologous region in different monocotyledons indicated that LABR_64 originates after the separation of the monocotyledonous and dicotyledonous species, and at the early differentiation stage of monocotyledons.【Conclusion】The rice blast resistance phenotypes are closely correlated with the presence of the LABR_64 locus in japonica subpopulation, but not in indica, indicating that there are many other resistance loci in the indica subpopulation. The LABR_64-2 sequence can be used for developing molecular markers in marker-assisted rice blast resistance breeding. It also indicated that LABR_64 may play a role in disease resistance in different monocotyledons.
Key words： rice; rice blast resistance gene; sequence diversity; gene origin
Yufei DENG, Minghao LIU, Dan WANG, Shimin ZUO, Houxiang KANG, Guoliang WANG . Origin, Distribution and Sequence Diversity of Rice Blast Resistance Locus LABR_64 in Rice[J]. Chinese Journal OF Rice Science, 2019 , 33(1) : 20 -27 . DOI: 10.16819/j.1001-7216.2019.8017
| [1] | Jiang N, Liu X L, Dai L Y, Wang G L.Advances on the mapping and cloning of blast resistance gene in rice.Chin Agric Sci Bull, 2010, 26(10): 270-275. |
| [2] | He X Y, Wang L, Wu W H, Chen Z M, Lin F, Chen Y S, Liu W, Chen Y H, Liao Y P.The progress of mapping, isolation of the genes resistance to blast and their breeding application in rice.Chin Agric Sci Bull, 2014, 30(6): 1-12. |
| [3] | Dangl J L, Jones J D G. Plant pathogens and integrated defense responses to infection.Nature, 2001, 411(6839): 826-333. |
| [4] | Iyer A S, Mccouch S R.The rice bacterial blight resistance gene xa5 encodes a novel form of disease resistance. Mol Plant-Microbe Int, 2004, 17(12): 1348-1354. |
| [5] | Kobe B, Deisenhofer J.A structural basis of the interactions between leucine-rich repeats and protein ligands.Nature, 1995, 374(6518): 183-186. |
| [6] | Tameling W I, Elzinga S D, Darmin P S, Vossen J H, Takken F L, Haring M A, Cornelissen B J.The tomato R gene products I-2 and MI-1 are functional ATP binding proteins with ATPase activity.Plant Cell, 2002, 14(11): 2929. |
| [7] | Zhang X, Yang S, Wang J, Jia Y, Huang J, Tan S, Zhong Y, Wang L, Gu L, Chen J Q, Pan Q, Bergrelson J, Tian D.A genome-wide survey reveals abundant rice blast R genes in resistant cultivars. Plant J, 2015, 84(1): 20-28. |
| [8] | Wang Z X, Yano M, Yamanouchi U, Iwamoto M, Hayasaka H, Katayose Y, Sasaki T.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes. Plant J, 1999, 19(1): 55-64. |
| [9] | Bryan G T, Wu K S, Farrall L, Jia Y, Hershey H P, McAdams S A, Faulk K N, Donaldson G K, Tarchini R, Valent B. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta. Plant Cell, 2000, 12(11): 2033-2046. |
| [10] | Zhou B, Qu S, Liu G, Dolan M, Sakai H, Lu G, Bellizzi M, Wang G L.The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea. Mol Plant-Microbe Int, 2006, 19(11): 1216-1228. |
| [11] | Qu S H, Liu G F, Zhou B, Bellizzi M, Zeng L R, Dai L Y, Han B, Wang G L.The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice. Genetics, 2006, 172(3): 1901-1914. |
| [12] | Lee S K, Song M Y, Seo Y S, Kim H K, Ko S, Cao P J, Suh J P, Yi G, Roh J H, Lee S, An G, Hahn T R, Wang G L, Ronald P, Jeon J S.Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes. Genetics, 2009, 181(4): 1627-1638. |
| [13] | Fukuoka S, Saka N, Koga H, Ono K, Shimizu T, Ebana K, Hayashi N, Takahashi A, Hirochika H, Okuno K, Masahiro Yano.Loss of function of a proline-containing protein confers durable disease resistance in rice.Science, 2009, 325(5943): 998-1001. |
| [14] | Lin F, Chen S, Que Z Q, Wang L, Liu X Q, Pan Q H.The blast resistance gene Pi37 encodes a nucleotide binding site-leucine-rich repeat protein and is a member of a resistance gene cluster on rice chromosome 1. Genetics, 2007, 177(3): 1871-1880. |
| [15] | Ashikawa I, Hayashi N, Yamane H, Kanamori H, Wu J Z, Matsumoto T, Ono K, Yano M.Two adjacent nucleotide-binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance. Genetics, 2008, 180(4): 2267-2276. |
| [16] | Takahashi A, Hayashi N, Miyao A, Hirochika H.Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging. Bmc Plant Biol, 2010, 10(1): 175-186. |
| [17] | Zhai C, Lin F, Dong Z Q, He X Y, Yuan B, Zeng X S, Wang L, Pan Q H.The isolation and characterization of Pik, a rice blast resistance gene which emerged after rice domestication. New Phytol, 2011, 189(1): 321-334. |
| [18] | Deng Y, Zhai K, Xie Z, Yang D, Zhu X, Liu J, Wang X, Qin P, Yang Y, Zhang G, Li Q, Zhang J, Wu S, Millazzo J, Mao B, Wang E, Xie H, Tharreau D, He Z.Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance.Science, 2017, 355(6328): 962-966. |
| [19] | Yi G, Lee S K, Hong Y K, Cho Y C, Nam M H, Kim S C, Han S S, Wang G L, Hahn T R, Ronald P C, Jeon J S.Use of Pi5(t) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea. Theor Appl Genet, 2004, 109(5): 978-985. |
| [20] | Jeon J S, Chen D, Yi G H, Wang G L, Ronald P C.Genetic and physical mapping of Pi5(t), a locus associated with broad-spectrum resistance to rice blast. Mol Genet Genom, 2003, 269(2): 280-289. |
| [21] | Inukai T, Zeigler R S, Sarkarung S, Bronson M, Dung L V, Kinoshita T, Nelson R J.Development of pre-isogenic lines for rice blast-resistance by marker-aided selection from a recombinant inbred population.Theor Appl Genet, 1996, 93(4): 560-567. |
| [22] | Kang H X, Wang Y, Peng S S, Zhang Y L, Xiao Y H, Wang D, Qu S H, Li Z Q, Yan S Y, Wang Z L, Liu W D, Ning Y S, Korniliev P, Leung H, Mezey J, McCouch S R, Wang G L. Dissection of the genetic architecture of rice resistance to the blast fungus Magnaporthe oryzae. Mol Plant Pathol, 2015, 17(6): 959-972. |
| [23] | ParK C H, Wang G L. The Magnaporthe oryzae effector AvrPiz-t Targets the RING E3 ubiquitin ligase APIP6 to suppress pathogen-associated molecular pattern-triggered immunity in rice. Plant Cell, 2012, 24(11): 4748-4762. |
| [24] | Wang B H, Zhen W, Lu G D, Zhang X B, Wang Z H.Screening on the spore-producing media ofPyricularia oryzae. Fujian Agric Sci Technol, 2000(2): 1-2. |
| [25] | Huang X H, Yang S H, Gong J Y, Zhao Q, Feng Q, Zhan Q L, Zhao Y, Li W J, Cheng B Y, Xia J H, Chen N, Huang T, Zhang L, Fan D L, Chen J Y, Zhou C C, Lu Y Q, Weng Q J, Han B.Genomic architecture of heterosis for yield traits in rice.Nature, 2016, 537(7622): 629-633. |
| [26] | Vogel J P, Garvin D F, Mockler T C.Genome sequencing and analysis of the model grass Brachypodium distachyon. Nature, 2010, 463(7282):763-768. |
| [27] | Zhang L, Yu H, Ma B, Liu G F, Wang J J, Wang J M, Gao R C, Li J J, Liu J Y, Xu J, Zhang Y Y, Li Q, Huang X H, Xu J L, Li J M, Qian Q, Han B, He Z H, Li J Y.A natural tandem array alleviates epigenetic repression of IPA1 and leads to superior yielding rice.Nat Commun, 2017, 8(14789): 1-14. |
| [28] | Marschalek R, Silva M C, Santos S B D, Manke J R, Bieging C, Porto G, Wickert E, Andrade A D. Image-Rice Grain Scanner: A three-dimensional fully automated assessment of grain size and quality traits.Crop Breed Appl Biotechnol, 2017, 17: 89-97. |
| [29] | Luo C P, Ni L, Chen Z Y, Liu Y F, Liu Y Z, Nie Y F. Inoculation technology of rice blast and rice resistance to it in Jiangsu regional tests in 2009. Jiangsu Agric Sci, 2009(6): 178-179. |
| [30] | Zhu Y Y, Chen H, Wang Y Y, Li Z S, Li Y, Fan J H, Chen J B, Yang S S, Ma G L, Hu L P, Zou J Y, Mundt C C, Borromeo E, Leung H, Mew T W.Diversifying variety for the control of rice blast in China.Biodiversity, 2001, 2(1): 10-14. |
| [31] | Lei C L, Wang J L, Jiang W R, Ling Z Z, George M L.Population structure and genetic variation of rice blast fungus in some rice-growing regions in northern China.Acta Phytopathol Sin, 2002, 32(3): 219-226. |
| [32] | Wang G L, Mackill D J, Bonman J M, McCouch S, Champoux M C, Nelson R J. RFLP Mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar.Genetics, 1994, 136(4): 1421-1434. |
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