Chinese Journal OF Rice Science >
Screening for Strains of Rice Spikelet Rot Disease Pathogenic Fungus with High Fumonisin Production and Strong Pathogenicity
Received date: 2018-04-16
Revised date: 2018-06-25
Online published: 2018-11-10
【Objective】 The objective is to develop a stable and efficient artificial inoculation technique to identify the pathogenicity of different strains for rice spikelet rot disease (RSRD), and to test the ability of F. proliferatum to synthesize fumonisin in two culture media by detecting and analyzing the contents of fumonisin. Based on the methods, strains with high fumonisin production and strong pathogenicity were selected. 【Method】 Injection and spraying inoculation methods were adopted at booting and heading stages. The pathogenicity and stability of different inoculation methods at different growing stage of rice were investigated. The pathogenicity and the ability of fumonisin production of different strains were investigated by using suitable inoculation methods and HPLC-MS/MS analysis. 【Result】 The incidence of RSRD is high and stable with injection inoculation at pollen mother cell meiosis to maturing stages, and rice grain yield was significantly affected by inoculation during pollen mother cell formation to initial heading stage. The high pathogenic strains FP4, FP6, FP8, FP9 and FP10 were selected based on injection inoculation. The fumonisin synthesis assaying was conducted by using HPLC-MS/MS, and the strains FP4 and FP9, with high fumonisin production and strong pathogenicity were identified finally. The culture media made of rice grains was more suitable than that making of corn for fumonisin synthesis.【Conclusion】 The invasion period of F. proliferatum of RSRD was pollen mother cell formation to full heading stage, the initial infection stage was pollen mother cell formation to meiosis stage, and the effect of RSRD on rice yield was closely related to infection stage.
Lei SUN, Ling WANG, Lianmeng LIU, Yuxuan HOU, Qiqin LI, Shiwen HUANG . Screening for Strains of Rice Spikelet Rot Disease Pathogenic Fungus with High Fumonisin Production and Strong Pathogenicity[J]. Chinese Journal OF Rice Science, 2018 , 32(6) : 610 -616 . DOI: 10.16819/j.1001-7216.2018.8045
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