Chinese Journal OF Rice Science >
Map-based Cloning of Female Abortion (FA) Gene in Rice
Received date: 2018-03-09
Revised date: 2018-04-03
Online published: 2018-11-10
【Objective】Phenotypic observation, genetic mapping and gene cloning of rice embryo sac mutants will lay a theoretical basis for recovering plant reproductive development.【Method】A female abortion (fa) mutant was obtained from ethyl methylsulfonate (EMS)-treated Ningjing 4. The embryo sacs of the wild-type and mutant were observed at different developmental stages, and the types of mutant embryo sacs were also calculated. The heterozygous mutant was used as the female parent and N22 as the male parent to construct the mapping population. The phenotype was analyzed by genetic analysis, and the target gene was precisely mapped by map-based cloning method. 【Result】Phenotypic analysis showed that the mutant’s pistil showed no difference in appearance, but the plants showed complete sterility at maturity. Compared with the wild type, the development of the mutant embryo sacs was abnormal, which could not form seven-cell eight-nuclear structure. Genetic analysis showed that the fa mutant traits were controlled by a single recessive gene. The gene was initially mapped between the L1 and L3 markers on chromosome 1 and finally between markers L10 and L11 by expanding the mapping population, with a physical distance of 117 kb. Sequencing analysis showed that LOC_Os01g68870 had a single base substitution in exon, from thymine(T) to cytosine(C), leading to amino acid change from leucine to proline and this phenotype. The results of qRT-PCR showed that compared with OsMADS13, OsAPC6 and OsTDL1A, the expression level of OsDEES1 in the mutant fa embryo sacs was the most significant, and FA might act at OsDEES1 upstream. Subcellular localization showed that the FA protein is localized to the plasma membrane. 【Conclusion】 According to the present study, FA gene was a new allele of MSP1. Our work further elucidates the importance of this gene in the development of embryo sacs and provides new clues in regulation the network system.
Key words： female abortion mutant; genetic analysis; map-based cloning; rice
Feng LÜ, Qianying TANG, Qiming WANG, Hai ZHENG, Shimin YOU, Wenting BAI, Yanjia XIAO, Zhigang ZHAO, Jianmin WAN . Map-based Cloning of Female Abortion (FA) Gene in Rice[J]. Chinese Journal OF Rice Science, 2018 , 32(6) : 519 -528 . DOI: 10.16819/j.1001-7216.2018.8023
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