Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2018 , Vol. 32 >Issue 5: 493 - 500

DOI: https://doi.org/10.16819/j.1001-7216.2018.7119

实验技术

Detection of Seed-borne Rice Bakanae Pathogens in Jiangsu Province, China Using Loop-mediated Isothermal Amplification Assays

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  • 1 College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
    2College of Agronomy, Nanjing Agricultural University, Nanjing 210095, China
    3Zhenjiang Agric-academy of Sciences, Zhenjiang 212400, China
*Corresponding author, E-mail: xbzheng@njau.edu.cn

Received date: 2017-09-26

  Revised date: 2017-12-13

  Online published: 2018-09-10

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Abstract

【Objective】The research aims to reveal the situation of seed-borne rice bakanae pathogens in Jiangsu Province, China. 【Methods】The seeds of 65 major rice cultivars collected from this area were analyzed by using loop-mediated isothermal amplification (LAMP) assays for specific detection of 4 known rice bakanae pathogen species, including Fusarium fujikuroi, F. proliferatum, F. verticilliodes and F. andiyazi. 【Results】There were 54 rice cultivars carrying rice bakanae pathogens, indicating it’s a common phenomenon that rice seeds carrying rice bakanae pathogens in Jiangsu Province. And the most frequent species was F. fujikuroi, representing 83.07% of the total samples tested, followed by F. proliferatum and F. andiyazi, representing 10% and 6% respectively. However, none of the 65 rice cultivars was tested to be carrying F. verticilliodes. 【Conclusions】The method is readily available for detecting seed-borne rice bakanae pathogens.

Key words： rice bakanae; detection of seed-borne pathogens; loop-mediated isothermal amplification

Cite this article

Yongtian YUAN, Zhenyang RONG, Wenwu YE, Hongsheng ZHANG, Yiqing ZHUANG, Xiaobo ZHENG . Detection of Seed-borne Rice Bakanae Pathogens in Jiangsu Province, China Using Loop-mediated Isothermal Amplification Assays[J]. Chinese Journal OF Rice Science, 2018 , 32(5) : 493 -500 . DOI: 10.16819/j.1001-7216.2018.7119

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