Chinese Journal OF Rice Science >
The Guanylate Kinase OsGK1 is Essential for Seed Development in Rice
Received date: 2018-01-15
Revised date: 2018-03-17
Online published: 2018-09-10
【Objective】In this study, the phenotype of the floury and shrunken endosperm mutant fse2 was analyzed. Isolation of the responsible gene will lay a foundation for elucidating the mechanism underlying starch synthesis and embryo development in rice. 【Method】fse2 was obtained from the mutant library of japonica cultivar Dianjingyou 1 induced with N-Nitroso-N-methylurea. In this study, the physiochemical properties of fse2 endosperm were investigated and the structure of starch grains was observed. An F2 population derived from fse2 and N22 was constructed, then the underlying gene was determined by map-based cloning and complementation tests. qRT-PCR and GUS staining were used to analyze the expression of FSE2. Western blotting was performed to analyze the protein levels of starch synthesis related genes and mitochondrial genes in the mutant. 【Result】Compared with the transparent endosperm of wild type, fse2 displayed a floury and shrunken endosperm, significantly declined 1000-grain weight, smaller and loosely packed irregular compound starch grains. Total starch and amylose content decreased significantly, while the lipid content increased obviously in fse2, and the gelatinization characteristics of fse2 were changed notably. FSE2 encodes a guanylate kinase named OsGK1, which is dual-targeted to both mitochondria and plastids. OsGK1 was constitutively expressed in various organs with the highest level in developing endosperm at 6th day after flowering. The protein levels of most of the starch synthesis related genes in the mutant endosperm were significantly decreased, especially AGPS2b and PHOI. In addition, the homozygous seeds of fse2 were lethal and the development of fse2 embryos was severely arrested. The accumulation of mitochondria AOX was notably elevated, while almost undetectable in the wild type, indicating the mitochondrial respiratory chain was impaired. 【Conclusion】Due to the functional defects of OsGK1, the development of mitochondria and amyloplasts are abnormal, leading to the embryo lethality and a floury and shrunken endosperm.
Jingfang LI, Yunlu TIAN, Xi LIU, Shijia LIU, Liangming CHEN, Ling JIANG, Wenwei ZHANG, Dayong XU, Yihua WANG, Jianmin WAN . The Guanylate Kinase OsGK1 is Essential for Seed Development in Rice[J]. Chinese Journal OF Rice Science, 2018 , 32(5) : 415 -426 . DOI: 10.16819/j.1001-7216.2018.8003
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