Chinese Journal OF Rice Science >
Isolation and Characteristic Analysis of Protein Kinase Promoter OsSRLp from Rice
Received date: 2017-10-26
Revised date: 2017-12-28
Online published: 2018-07-10
【Objective】To study the construction and function of OsSRL, a typeⅠcasein kinase, 【Method】OsSRL and about 1600 bp up-stream of the gene named as OsSRLp were isolated using genomic DNA of rice Zhonghua 11. The regulatory elements of OsSRLp were analyzed using online software of PLACE, and the results showed that several key cis-elements which associated with reproductive process, signal and stress response were included. 【Results】The expression pattern analysis showed that OsSRL was constitutively expressed in different tissues of rice. The fusion expression vector of OsSRLp and GUS was constructed, and the vector was transformed into rice callus. After positive transgenic lines were obtained, the GUS activities were further detected, and the results showed that relative higher level expression was detected in spikelet, root and stem, as well as low expression levels in other rice tissues. The results indicated that OsSRLp possess constitutive activities with slightly variations in different rice tissues. 【Conclusion】 Combined with the characteristic elements and their potential functions of OsSRLp, the results implied that OsSRL and its promoter OsSRLp could participate in and regulate the development and reproduction progresses of plants.
Qingguo WANG, Yingying WANG, Zhen LI, Jiaowen PAN, Yifan WANG, Yingxiu LI, Wei LIU . Isolation and Characteristic Analysis of Protein Kinase Promoter OsSRLp from Rice[J]. Chinese Journal OF Rice Science, 2018 , 32(4) : 335 -341 . DOI: 10.16819/j.1001-7216.2018.7127
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