Chinese Journal OF Rice Science >
Targeted Editing of Rice SD1 Gene UsingCRISPR/Cas9 System
Received date: 2017-09-01
Revised date: 2017-12-27
Online published: 2018-05-10
【Objective】In the last century, the usage of semi-dwarfrice varieties brought about significant improvements and great achievements inrice breeding.Most of the semi-dwarfrice varieties have loss-of-function mutations in the Semi-Dwarf1 (SD1) gene.In order to obtain sd1 mutant, targeted editing of SD1 gene was performed. 【Method】SD1 gene editing vector CRISPR-SD1 was constructed by using CRISPR/Cas9 system, and transformed into two rice restore linesShenfan17 and Shenfan24 by the Agrobacterium-mediated method.【Result】 Homozygous sd1 mutants were obtained in T0 generation in both varieties, and vector-free sd1 mutant lines were segregated from the T1 population. The plant heights of the two sd1 mutants were decreased by 25% as compared with those of their wild types. 【Conclusion】CRISPR/Cas9 is a powerful tool for rice target gene editing, and has enormous potential in rice molecular breeding.
Key words： CRISPR/Cas9; gene editing; rice; Semi-Dwarf1
Xuejiao HU, Jia YANG, Can CHENG, Jihua ZHOU, Fuan NIU, Xinqi WANG, Meiliang ZHANG, Liming CAO, Huangwei CHU . Targeted Editing of Rice SD1 Gene UsingCRISPR/Cas9 System[J]. Chinese Journal OF Rice Science, 2018 , 32(3) : 219 -225 . DOI: 10.16819/j.1001-7216.2018.7106
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