Chinese Journal OF Rice Science >
Development and Verification of a Functional Marker Associated with Resistance to ALS Inhibitor Herbicide
Received date: 2017-08-01
Online published: 2018-03-10
【Objective】Breedingrice varieties with resistance to ALS-inhibiting herbicide is the mosteconomical and effective method to preventweeds in direct-sowing field, and molecular marker-assisted selection canimprove its efficiency. 【Method】The dominant molecular marker, designed for genotypic detection in different varieties and an F2 population derived fromHuaidao5/Huanghuazhan M-1(mutant with resistance to ALS-inhibiting herbicide) was developed with Tetra-primer ARMS-PCR technique, based on the clarified mutation in CDS of ALS gene. 【Result】 The sequence alignment analysis of ALS gene encoding regionshowed that only the mutation from T1642G1643 to A1642T1643 leading to the 548th amino acid substitutionfrom tryptophan to methioninein highly conserved regionwas the critical factors to produce resistance to ALS-inhibiting herbicide inHuanghuazhan M-1, despitethe existence of multiplebase differencesbetween different indica and japonica rice varieties.The results of PCR detection indicated that three different genotypescould be accurately distinguished by electrophoretic bands, and it was exactly consistent with the phenotype of herbicide resistance at the seedling stage. 【Conclusion】 The genotype associated with two continuous base mutations could be rapidly detected by Tetra-primer ARMS-PCRtechnique, and the efficiency for varieties with resistance to ALS-inhibiting herbicidecould be improved in breeding process.
Tao CHEN, Shanlei ZHANG, Ling ZHAO, Yadong ZHANG, Zhen ZHU, Qingyong ZHAO, Lihui ZHOU, Shu YAO, Chunfang ZHAO, Wenhua LIANG, Cailin WANG . Development and Verification of a Functional Marker Associated with Resistance to ALS Inhibitor Herbicide[J]. Chinese Journal OF Rice Science, 2018 , 1(1) : 137 -145 . DOI: 10.16819/j.1001-7216.2018.7091
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