Chinese Journal OF Rice Science >
Effects of Exogenous Ethephon on Fertility of Thermo-sensitive Genic Male Sterile Rice Lines Under Low Temperature Inducement
Received date: 2017-04-19
Online published: 2018-03-10
【Objective】Thisresearch aims toelucidate the mechanism behind sterility conversion(infertile convert into fertile)in low temperatureofthermo-sensitive genic male sterile ricein seed production in South China by applying exogenous ethephon.【Method】The thermo-sensitive genic male sterile rice cultivars Zhu 1S and Zhun S weretreated with artificial cooling water tank (low temperature, 22.5℃) induction for 7 days and sprayed withethephon (which promote synthesis of ethylene) and the chlorine dioxide solution (inhibitory substances)during fertility-sensitive period.Then the pollendyeing rate and the baggedseed-setting rate were calculatedand the quantitative real-time PCR was conducted.In addition, the promoters of OsACO5 and OsACS5, which are key enzymes of ethylene synthesis pathway, were isolated and analyzed.【Result】Under22.5℃for 7 days, the pollen rate of Zhu 1S and Zhun Swas 4.3% and 8.9%, and bagged seed-setting rate was 1.6% and 2.5%, respectively. Compared with the water spray control, the pollen rate of lines Zhu 1S and Zhun S was reduced by 3.3and 7.2 percent, respectively when spraying with 1600mg/L ethephon. When spraying with 1500 mg/L of chlorine dioxide solution, the pollen rate was increased by 3.5and 3.3 percent, and the bagged seed setting rate increased by 0.78and 0.52 percent, respectively. The expression of OsACO5 and OsACS5in young panicles was significantly increased at 1600 mg/L of ethephon under 22.5℃ inductionfor 7 days, which were 1.19, 1.24 (Zhu 1S) and 1.24, 1.1 (Zhun S) times of that of the control (water spray) respectively. When sprayed with 1500mg/L of chlorine dioxide solution for 7 days, the expression of OsACS5 gene in the Zhu 1S and Zhun S was significantly reduced, which were 0.93 and 0.89 times of that of the control (water spray), respectively, but the expression of OsACO5 was not significantly changed. Compared with normal field temperature (control) for 7 days, water spraying, 1600 mg/L of ethephon and 1500 mg/L of chlorine dioxide solution at22.5℃down-regulated the expression of OsACO5 and OsACS5 in young panicles of Zhu 1S and Zhun S.The promoters of OsACO5 and OsACS5contained multiple tissue-specific expression elements and ethylene-regulated cis-acting elements in addition to the basic structures such as TATA-box and CAAT-box. 【Conclusion】Under low temperature condition, spraying 1600mg/L ethephon in the sensitive period of thermo-sensitive genic male sterile rice had inhibitory effect on fertility conversion from sterility to fertility.
Linzhi LI, Jiaping WEI, Yanfeng JIA, Xiaolin LIU, Haiqing ZHANG, Ming WANG, Hao MA . Effects of Exogenous Ethephon on Fertility of Thermo-sensitive Genic Male Sterile Rice Lines Under Low Temperature Inducement[J]. Chinese Journal OF Rice Science, 2018 , 1(1) : 128 -136 . DOI: 10.16819/j.1001-7216.2018.7045
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