Chinese Journal OF Rice Science >
QTL Analysis for Mineral Contents in Brown Rice Using a BC2F4:5 Population Derived from Dongxiang Wild Rice (Oryza rufipogon Griff.)
Received date: 2017-01-19
Revised date: 2017-04-02
Online published: 2018-01-10
【Objective】 Biofortifying food crops with essential minerals would alleviate mineral deficiencies in humans.【Method】 One plant A58 was selected from Xieqingzao B// Xieqingzao B /Dongxiang wild rice BC1F5 population, and was backcrossed with Xieqingzao B to develop a BC2F4:5 population. The contents of Mg, Ca, Zn, Fe, Mn and Cu in brown rice of 132 BC2F4:5 lines were measured with an inductively coupled plasma atomic emission spectrometer (ICP-AES). Detection of quantitative trait loci (QTLs) for mineral contents in brown rice was conducted using Windows QTL Cartographer 2.5.【Result】 A total of 17 QTLs for mineral contents in brown rice were detected on chromosomes 1, 4, 6, 8, 9 and 11, respectively, including one for Mg content, four for Ca content, four for Zn content, two for Fe content, two for Mn content, and four for Cu content. The explained phenotypic variations ranged from 5.0% to 47.2%, and eight QTLs of them had the enhancing alleles derived from Oryza rufipogon. 【Conclusion】 Twelve QTLs were clustered in five chromosomal regions, indicating that common genetic-physiological mechanisms were involved for different mineral nutrients, and the beneficial alleles could be utilized to improve grain nutritional quality by marker-assisted selection.
Key words： Dongxiang wild rice; brown rice; mineral content; QTL mapping
Biaolin HU, Derun HUANG, Yeqing XIAO, Qiangsheng HE, Yong WAN, Yeyang FAN . QTL Analysis for Mineral Contents in Brown Rice Using a BC2F4:5 Population Derived from Dongxiang Wild Rice (Oryza rufipogon Griff.)[J]. Chinese Journal OF Rice Science, 2018 , 32(1) : 43 -50 . DOI: 10.16819/j.1001-7216.2018.7011
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