Chinese Journal OF Rice Science >
Overexpression of OsENO2-2 Affects Heading Date in Rice
Received date: 2017-05-18
Revised date: 2017-09-15
Online published: 2018-01-10
【Objective】 OsENO2-2 was isolated from japonica cultivar Zhonghua 11 as a short transcript produced by alternative splicing of OsENO2 which is homologous to AtENO2. To reveal the function of OsENO2-2,【Method】 we constructed an overexpression vector of OsENO2-2 and obtained the transgenetic plants. Based on the observation and statistics of phenotype in overexpressed plant and wild-type, we validated the function of OsENO2-2 using reverse genetics method. 【Result】 Overexpression of OsENO2-2 transgenetic plants delayed heading date only under long-day conditions but not short-day conditions. qRT-PCR expression analysis of the critical flowering time genes showed that in long-day conditions, the expression of RFT1 in overexpressed plants was downregulated, and the expression levels of the other tested genes did not change significantly in wild-type and overexpressed plants; but in short-day conditions, the expression of all the tested genes in wild-type and over-expression plants did not change significantly. 【Conclusion】 OsENO2-2 might be involved in the photoperiodic pathways to regulate the heading date in rice by regulating the expression of RFT1 gene.
Key words： rice; overexpression; OsENO2-2; enolase gene; heading date
Xiaoyun LIU, Xiao LI, Tengfei LI, Lufang SU . Overexpression of OsENO2-2 Affects Heading Date in Rice[J]. Chinese Journal OF Rice Science, 2018 , 32(1) : 12 -22 . DOI: 10.16819/j.1001-7216.2018.7055
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