Chinese Journal OF Rice Science >
Expression Patterns of OsHKT Genes in Rice
Received date: 2017-06-08
Revised date: 2017-07-07
Online published: 2017-11-10
【Objective】OsHKTs (High Affinity K+ Transporter) encode Na+ or K+ transporter or Na+-K+ co-transporters that play an important role in Na+ recirculation and maintaining the low concentration of Na+ and low Na+ / K+ ratio in above-ground tissues, especially leaves. Therefore, OsHKT family is related to salt stress tolerance in rice. In this study, we analyzed the tissue specificity and diurnal rhythm of OsHKTs gene expression and expression patterns in rice seedlings treated with either salt or ABA. 【Method】qRT-PCR technique was used to analyze OsHKTs expression patterns. 【Result】Individual member of OsHKT family genes had different expression levels in root and leaf. OsHKT1;1, OsHKT1;3, OsHKT2;3, and OsHKT2;4 mainly expressed in leaves, whereas other members had higher transcript levels in roots relative to those in leaves. Salt treatment affected transcript levels of OsHKT family genes both in leaves and roots. OsHKT1;5, OsHKT2;1, and OsHKT2;2 had similar expression patterns in both leaves and roots of seedlings treated with NaCl, whereas other members did not. Transcript levels of OsHKT family genes were regulated by ABA treatment either in leaves or in roots. OsHKT1;3 and OsHKT1;5 had similar expression patterns in both leaves and roots. In addition, expression patterns of OsHKT genes exhibited similar diurnal rhythm. 【Conclusion】Our results suggest that expression of OsHKT family genes has tissue-specificity and similar diurnal rhythm. Exogenous NaCl and ABA treatment have different influences on OsHKT transcripts, depending on individual member of the family. Our findings lay a foundation for further revealing the functional mechanism of OsHKT genes in rice.
Key words： OsHKT; expression patterns; salt stress; ABA; tissue specificity; photoperiod
Lixin CUI, Yanan HE, Yaping LI, Xianzhi XIE . Expression Patterns of OsHKT Genes in Rice[J]. Chinese Journal OF Rice Science, 2017 , 31(6) : 559 -567 . DOI: 10.16819/j.1001-7216.2017.7070
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