Chinese Journal OF Rice Science >
Functional Analysis of MoMET3 in Growth, Development and Pathogenicity of Magnaporthe oryzae
Received date: 2017-02-13
Revised date: 2017-05-23
Online published: 2017-09-10
【Objective】 Sulfur is generally made biologically available by activation with ATP in a reaction catalyzed by ATP sulfurylase in fungi. The function of ATP sulfurylase in the growth and pathogenecity of Magnaporthe oryzae was analyzed to lay a foundation for target determination for rice blast prevention.【Method】The knockout vector was constructed by homologous recombination method, and the phenotype of the mutant was analyzed.【Result】MoMET3 was required for vegetative growth and conidiation, but not a virulence factor. The growth rate and conidiation of MoMET3- deleted mutants were significantly decreased in complete medium. Loss of MoMET3 did not affect conidial germination and appressiorial formation, but the growth of secondary hyphae was inhibited in the minimal medium. After complementation of MoMET3 gene, the mutant returned to normal growth and can utilize sulfate. 【Conclusion】These evidences revealed that the reduction of inorganic sulfur is not necessary for the process of conidial germination and appressiorial formation. The endogenous reduced sulfur in conidia was sufficient to meet the sulfur needs in the process of conidial germination and appressiorial formation, and utilization of host-derived reduced sulfur played an important role in the hypha expansion of the blast fungus in hosts.
Key words： Magnaporthe oryzae; ATP sulfurylase; MoMET3; functional analysis
Xiangyang FENG, Zhen ZHANG, Rongyao CHAI, Haiping QIU, Jiaoyu WANG, Xueqin MAO, Yanli WANG, Guochang SUN . Functional Analysis of MoMET3 in Growth, Development and Pathogenicity of Magnaporthe oryzae[J]. Chinese Journal OF Rice Science, 2017 , 31(5) : 542 -550 . DOI: 10.16819/j.1001-7216.2017.7018
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