Chinese Journal OF Rice Science >
Function Analyses of Rice Nitrate Transporter Gene OsNPF7.9 in Nitrogen Accumulation and Transport
Received date: 2017-03-15
Revised date: 2017-04-06
Online published: 2017-09-10
【Objective】 NPF family in plants can transport nitrate and peptide, etc. Functional analyses of OsNPF7.9 can lay a theoretical basis for studying molecular mechanism of high nitrogen use-efficiency.【Method】 Bioinformatics of OsNPF7.9 was predicted with different biological softwares. The OsNPF7.9 promoter-GUS transgenic rice was obtained for analyzing the spatial expression pattern of OsNPF7.9. And the expression patterns of OsNPF7.9 under different N supply levels were observed using semi-quantitative RT-PCR and pOsNPF7.9::GUS transgenic rice. OsNPF7.9 overexpressing rice was obtained, and their different physiological indexes were measured. 【Result】 OsNPF7.9 is localized on plasma membrane with 12 transmembrane domains and a big hydrophilic loop between the 6th and the 7th transmembrane domain. Tissue expression pattern showed that OsNPF7.9 was expressed in roots, leaves, root-shoot junction and flowers. Both RT-PCR and GUS staining of pOsNPF7.9::GUS transgenic rice indicated that the expression of OsNPF7.9 was not affected by N concentration and form. Overexpression of OsNPF7.9 could significantly increase not only nitrate concentration in shoots and roots-shoots nitrate ratio; but also total nitrogen concentration and accumulation in shoots, and roots-shoots total nitrogen ratio. 【Conclusion】OsNPF7.9 participated nitrate transport from roots to shoots, and overexpression of OsNPF7.9 could increase N accumulation and transport from roots to shoots.
Key words： rice; nitrogen; OsNPF7.9; nitrate transporter
Huimin FENG, Hong LU, Hanqing WANG, Xinyue LI . Function Analyses of Rice Nitrate Transporter Gene OsNPF7.9 in Nitrogen Accumulation and Transport[J]. Chinese Journal OF Rice Science, 2017 , 31(5) : 457 -465 . DOI: 10.16819/j.1001-7216.2017.7031 457
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