Chinese Journal OF Rice Science >
Effects of Urease Inhibitor and Nitrification Inhibitor on Nitrogen Transformation in Paddy Soil
Received date: 2017-01-17
Revised date: 2017-03-07
Online published: 2017-07-10
【Objective】The research is aimed to reveal the effects of urease inhibitor(UI) and nitrification inhibitor(NI) on nitrogen (N) transformation, and the fertilizer-saving mechanism of inhibitor(s). 【Method】 A field experiment was conducted in the middle and lower reaches of the Yangtze River area, Southern China with a randomized design and five treatments and three replicates: 1) CK (no N fertilizer), 2) U (urea only), 3) U+UI, 4) U+NI, 5) U+UI+NI. NBPT [N-(n-butyl) thiophosphoric triamide] and DMPP [3, 4-Dimethyl-1H-pyrazole phosphate] as the urease inhibitor and nitrification inhibitor was uniformly mixed with urea (U) at a rate of 10,000 mg/kg. The total urea, as base fertilizer, was applied to field before the transplanting of rice seedlings. The activities of urease and nitrate reductase, the contents of NH4+-N, NO3--N and microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN) in soil were analyzed in tillering and booting stages. The rice yield and N efficiency were investigated. The mechanism of increased yield and N efficiency due to inhibitor was elucidated by the stepwise regression analysis. 【Result】1) Compared with the normal urea treatment, NBPT addition and NBPT + DMPP significantly improved the grain yields and the recovery of applied N in the above-ground parts by 6.56% and 8.24%,19.4% and 23.7%, respectively. 2) The addition of NBPT and NBPT + DMPP in urea significantly reduced urease activity and soil NH4+-N content at the tillering stage, and increased soil NH4+-N content at the booting stage, without obvious effects on nitrate reductase activity (NRA), soil NO3--N content and microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN) in both stages. There is no significant difference in urease activity between urea and urea + NBPT at the booting stage. Therefore, the urease inhibitor NBPT was effective on inhibiting the activity of urease and improving the content of NH4+ -N before the booting stage. On the contrast, adding DMPP only had no obvious effects on these indexes above. 3)The stepwise regression analysis revealed that the grain yield of rice was significantly associated with NH4+-N content in soil at the tillering and booting stages, especially, the latter. However, other properties in soil have no obvious effects on the grain yield. 【Conclusion】Urea combined with NBPT and combination of NBPT + DMPP slowed down the hydrolytic action of urea and dramatically improved soil NH4+-N content in the booting stage, which is the dominate factor of improving the grain yields and the recovery of applied N in the above-ground parts. The conclusion is consistent with postponing nitrogen technique in agriculture.
Wenxue ZHANG, Chengchun YANG, Shaoxian WANG, Gang SUN, Zengbing LIU, Zuzhang LI, Guangrong LIU . Effects of Urease Inhibitor and Nitrification Inhibitor on Nitrogen Transformation in Paddy Soil[J]. Chinese Journal OF Rice Science, 2017 , 31(4) : 417 -424 . DOI: 10.16819/j.1001-7216.2017.7008 417
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