Chinese Journal OF Rice Science >
Development of Permanent Genic Male Sterile Line by Pyramiding Thermo-sensitive Genic Male Sterile Genes and Reverse Temperature Induced Genic Male Sterile Genes in Rice (Oryza sativa L.)
Received date: 2017-02-14
Revised date: 2017-03-30
Online published: 2017-07-10
： 【Objective】The security problem of seed production has seriously hampered the steadily and sustainable development of two-line hybrid rice. 【Method】Aizi S (thermos-sensitive genic male sterile, TSGMS) and Aiyan s (reverse temperature sensitive genic male sterile line, RTSGMS) were screened as the original parents to develop permanent genic sterile line (PGSL) according to the F1 fertility of crosses among 16 (light-) TSGMS and 4 RTSGMS. The fertility genes were introduced into the near isogenic lines Tianfeng S and Tianfeng s by backcrossing Aizi S and Aiyan s with the bridge parent Tianfeng B, respectively. Then, Tianfeng Ss, the PGSL, was observed from the cross between Tianfeng S and Tianfeng s. The fertility, restoration, competitive advantage of yield-related traits and reproductive characteristics of Tianfeng Ss were investigated. 【Result】 Tianfeng Ss was completely sterile under natural long day with high temperature and short day with low temperature. And no obvious difference was noted among the F1 crosses from Tianfeng Ss and its three parents and five restorers in spikelet fertility percentage. 【Conclusion】The results showed that the newly developed PGSL might solve the security problem of seed production in two-line hybrid rice fundamentally.
Jianhua JIANG, Jinlong NI, Shuang WU, Dezheng WANG . Development of Permanent Genic Male Sterile Line by Pyramiding Thermo-sensitive Genic Male Sterile Genes and Reverse Temperature Induced Genic Male Sterile Genes in Rice (Oryza sativa L.)[J]. Chinese Journal OF Rice Science, 2017 , 31(4) : 371 -378 . DOI: 10.16819/j.1001-7216.2017.7017 371
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