Chinese Journal OF Rice Science >
CRISPR/CAS9-mediated Editing of the Fragrant Gene Badh2 in Rice
Online published: 2017-03-10
【Objective】Fragrant rice, which is favored because of its quality characteristics of faint scent and tastiness, is a special rice type. Fragrance in rice is mainly controlled by the gene encoding betaine aldehyde dehydrogenase on chromosome 8.【Method】The fragrant gene Badh2 in Zhonghua 11 was edited by CRISPR/CAS9.【Result】Twenty T1 individuals derived from T0 generation were genotyped. One plant, which contains an additional T base in the first exon of Badh2 without the vector skeleton, was finally produced. qRT-PCR result suggested that Badh2 RNA level was decreased in the mutant. Compared with the wild type, the mutant increased 2-acetyl-1-pyrroline content by the GC-MS method. Furthermore, two rice yield-related traits including tiller numbers and seed-setting rate showed significant difference at the 0.05 level among five traits related to yield and three traits related to cooking and eating quality.【Conclusion】We succeed editing the Badh2 by CRISPR-CAS9 technical in rice, and it would provide abundant theoretical guidance and accelerate the breeding process of fragrant rice.
Key words： rice; Badh2; CRISPR/CAS9; fragrance
Gaoneng SHAO, Lihong XIE, Guiai JIAO, Xiangjin WEI, Zhonghua SHENG, Shaoqing TANG, Peisong HU . CRISPR/CAS9-mediated Editing of the Fragrant Gene Badh2 in Rice[J]. Chinese Journal OF Rice Science, 2017 , 31(2) : 216 -222 . DOI: 10.16819/j.1001-7216.2017.6098
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