Chinese Journal OF Rice Science >
A Comparative Study of Ozone-induced Leaf Injury of Rice with Different Ozone Sensitivity
Online published: 2017-03-10
【Objective】In order to provide reference for ozone-tolerant rice breeding, the leaf characteristics of rice with different ozone sensitivity in response to ozone stress were studied.【Method】By using glasshouse-type fumigation chambers, 23 rice cultivars or lines were grown in soil from around a week after transplanting to maturity at two ozone concentrations: low ozone concentration as control (C-O3, 10 nL/L) and high ozone concentration as elevated O3 treatment (E-O3, 100 nL/L). Based on the decrease in the above-ground biomass under high ozone concentration, these rice genotypes were clustered into three types by the MinSSw (dynamic clustering method-minimum sum of squares within groups) method, namely A, B and C in the order of ozone sensitivity from low to high, respectively. The effects of ozone stress on leaf bronzing score (LBS) and Soil and Plant Analyzer Development (SPAD) values of the top three leaves were examined, and the relationships between leaf LBS or SPAD under ozone stress and the ozone-induced changes in the above-ground biomass at maturity stage were studied.【Result】Ozone stress decreased the above-ground biomass of three rice types A, B and C at maturity by 19%, 39% and 52%, respectively, and significant treatment effects were detected in B and C. The LBS of rice under ozone stress increased along with the process of plant growth, but no significant difference was detected among different types of rice, irrespective of growth stages. Compared to the control, ozone stress significantly decreased the leaf SPAD values of all growth stages, and the decline was increased with the prolonging fumigation time and the lowering leaf position. Averaged across all growth stages, ozone stress significantly decreased the leaf SPAD values of the first, second and third leaf from the top by 11%, 18% and 30%, respectively. On the contrast, the influence of ozone stress on the leaf SPAD values showed no significant difference among different types of rice, and different growth stages showed the same trend. Correlation analysis showed that, although there were positive correlations between the ozone-induced changes in the rice above-ground biomass and LBS of a few growth stages, the ozone-induced changes in the above-ground biomass were not closely associated with that of the leaf SPAD values of all growth stages, and same trends were observed for all leaves at different leaf positions.【Conclusion】The above results indicated that under current experimental conditions, leaf bronzing score and leaf SPAD value were not suitable index to evaluate the rice tolerance to ozone stress.
Key words： rice; ozone; growth; leaf bronzing score; SPAD value
Zaisheng SHAO, Hairong MU, Yipeng ZHAO, Yilei JIA, Bin PENG, Lianxin YANG, Yunxia WANG . A Comparative Study of Ozone-induced Leaf Injury of Rice with Different Ozone Sensitivity[J]. Chinese Journal OF Rice Science, 2017 , 31(2) : 175 -184 . DOI: 10.16819/j.1001-7216.2017.6099
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