Chinese Journal OF Rice Science >
Identification and Gene Cloning of the Brittle Culm Mutant bc1-wu3 in Rice
Online published: 2017-03-10
【Objective】Culm strength, an important agronomic trait, is related to lodging resistance of rice. The research of brittle culm mutants and genes has great significance to demonstrate the regulation of genetic mechanism of culm mechanical strength and breed lodging resistance varieties.【Method】In this study, a brittle culm mutant, named bc1-wu3(brittle culm 1 from Wuyujing 3), was obtained from the japonica variety Wuyujing 3 after 60Co-γ induced mutagenesis. F2 population derived from bc1-wu3/Kasalath was used to mapping this corresponding gene by position cloning approach.【Result】The mutant was characterized by the brittle leaf, leaf sheath, and culm during the whole growing stage. Compared with the wild type, the panicle length and grain length of bc1-wu3 decreased significantly, and the grain width presented a significant increase. There was a significant decrease in the cellulose content in culm, the number of sclerenchyma cell layers and the thickness of sclerenchyma cell wall, but the contents of xylose, glucose and arabinose increased significantly in bc1-wu3. Genetic analysis showed that the brittle trait in bc1-wu3 was controlled by one single recessive nuclear gene, and the locus was mapped to a 57 kb genomic region on chromosome 3 between molecular markers M12 and Mk18. In the mapped region the cloned brittle culm gene BC1(LOC_Os03g30250) was included, which might be the candidate gene of bc1-wu3. Sequence analysis revealed that there was a single-base substitution(G-T) in the second exon of BC1 gene in bc1-wu3 mutant, leading to a residue substitution from cysteine to phenylalanine. The data from real-time RT-PCR indicated that there was significant decrease in the expression of BC1 gene in the culm of the mutant. 【Conclusion】Based on these results,we speculate that the bc1-wu3 gene is allelic to BC1 gene. These results would deepen our understanding for the function of BC1and help us to clarify the genetic mechanisms of culm strength.
Key words： Oryza sativa L.; brittle culm mutant; BC1 gene; genetic analysis; gene mapping
Zuopeng XU, Chongyuan ZHONG, Lijia ZHANG, Qiaoquan LIU . Identification and Gene Cloning of the Brittle Culm Mutant bc1-wu3 in Rice[J]. Chinese Journal OF Rice Science, 2017 , 31(2) : 157 -165 . DOI: 10.16819/j.1001-7216.2017.6130
| [1] | 张忠旭, 陈温福, 杨振玉, 华泽田, 高日玲, 高勇, 赵迎春. 水稻抗倒伏能力与茎秆物理性状的关系及其对产量的影响. 沈阳农业大学学报, 1999, 30(2): 81-85. |
| [1] | Zhang Z X, Chen W F, Yang Z Y, Hua Z T, Gao R L, Gao Y, Zhao Y C.Effect of lodging resistant on yield and its relationship with stalk physical characteristics.J Shenyang Agric Univ, 1999, 30(2): 81-85. (in Chinese with English abstract) |
| [2] | 马均, 马文波, 田彦华, 杨建昌, 朱开达, 周庆森. 重穗型水稻植株抗倒伏能力的研究. 作物学报, 2004, 30(2): 143-148. |
| [2] | Ma J, Ma W B, Tian Y H, Yang J C, Zhu K D, Zhou Q S.The culm lodging resistance of heavy panicle type of rice.Acta Agron Sin, 2004, 30(2): 143-148. (in Chinese with English abstract) |
| [3] | 刘畅, 李来庚. 水稻抗倒伏性状的分子机理研究进展. 中国水稻科学, 2016, 30(2): 216-222. |
| [3] | Liu CH, Li L G.Advance in molecular understanding of rice lodging resistance. Chin J Rice Sci, 2016, 30(2): 216-222. (in Chinese with English abstract) |
| [4] | 罗茂春, 田翠婷, 李晓娟, 林金星. 水稻茎秆形态结构特征和化学成分与抗倒伏关系综述. 西北植物学报, 2007, 27(11): 2346-2353. |
| [4] | Luo M C, Tian C T, Li X J, Lin J X.Relationship between morpho-anatomical traits together with chemical components and lodging resistance of stem in rice(Oryza sativa L .). Acta Bot Boreal, 2007, 27(11): 2346-2353. (in Chinese with English abstract) |
| [5] | 彭应财, 甘朝洪, 王鹤潼, 徐正进. 显性脆秆水稻不育系中脆A的选育. 杂交水稻, 2010, 25(4): 9-13. |
| [5] | Peng Y C, Gan C H, Wang H T, Xu Z J.Breeding of dominant brittle culm CMS line Zhongcui A in rice.Hybrid Rice, 2010, 25(4): 9-13. (in Chinese with English abstract) |
| [6] | 蒋钰东, 何沛龙, 廖红香, 张孝波, 吴国超, 何光华, 林婷婷, 桑贤春. 水稻茎秆脆性及叶尖枯死突变体fld1的鉴定与基因定位. 植物学报, 2014, 49(6): 663-671. |
| [6] | Jiang Y D, He P L, Liao H X, Zhang X B, Wu G C, He G H, Lin T T, Sang X C.Identification and gene mapping of a fragile and leaf-tip dead mutantfld1 in Oryza sativa. Chin Bull Bot, 2014, 49(6): 663-671. (in Chinese with English abstract) |
| [7] | Zhang B C, Liu X L, Qian Q, Liu L F, Dong G J, Xiong G Y, Zeng D L, Zhou Y H.Golgi nucleotide sugar transporter modulates cell wall biosynthesis and plant growth in rice.Proc Natl Acad Sci, 2011, 108: 5110-5115. |
| [8] | Zhou Y, Li S, Qian Q, Zeng D, Zhang M, Guo L, Liu X, Zhang B, Deng L, Liu X, Luo G,Wang X, Li J.BC10, a DUF266-containing and Golgi-located type II membrane protein, is required for cell-wall biosynthesis in rice(Oryza sativa L.).Plant J, 2009, 57: 446-462. |
| [9] | Xiong G Y, Li R, Qian Q, Song X Q, Liu X L, Yu Y, Zeng D L, Wan J, Li J, Zhou Y H.The rice dynamin-related protein DRP2B mediates membrane trafficking, and thereby plays a critical role in secondary cell wall cellulose biosynthesis.Plant J, 2010, 64(1): 56-70. |
| [10] | 舒亚洲, 曾冬冬, 秦冉, 金晓丽, 郑希, 石春海. 水稻脆秆突变体bc16的鉴定和基因精细定位. 中国水稻科学, 2016, 30(4): 345-355. |
| [10] | Shu Y Z, Zeng D D, Qin R, Jin X L, Zheng X, Shi C H.Identification and gene fine mapping of abrittle culm 16(bc 16) mutant in rice. Chin J Rice Sci, 2016, 30(4): 345-355. (in Chinese with English abstract) |
| [11] | Zhang B C, Deng L W, Qian Q, Xiong G Y, Zeng D L, Li R, Guo L B, Li J, Zhou Y H.A missense mutation in the transmembrane domain of CESA4 affects protein abundance in the plasma membrane and results in abnormal cell wall biosynthesis in rice. Plant Mol Biol, 2009, 71(4-5): 509-524. |
| [12] | Yan C J, Yan S, Zeng X H, Zhang Z Q, Gu M H.Fine mapping and isolation of Bc7(t), allelic to OsCesA4. J Genet Genom, 2007, 34(11): 1019-1027. |
| [13] | Tanaka K, Murata K, Yamazaki M, Onosato K, Miyao A, Hirochika H.Three distinct rice cellulose synthase catalytic subunit genes required for cellulose synthesis in the secondary wall.Plant Physiol, 2003, 133(1): 73-83. |
| [14] | Rao Y C, Yang Y L, Xin D D, Li X J, Zhai K E, Ma B J, Pan J W, Qian Q, Zeng D L.Characterization and cloning of a brittle culm mutant(bc88) in rice. Chin Sci Bull, 2013, 58(24): 3000-3006. |
| [15] | Kotake T, Aohara T, Hirano K, Sato A, Kaneko Y, Tsumuraya Y, Takatsuji H, Kawasaki S.RiceBrittle culm 6 encodes a dominant-negative form of CesA protein that perturbs cellulose synthesis in secondary cell walls. J Exp Bot, 2011, 62(6): 2053-2062. |
| [16] | Zhang M, Zhang B C, Qian Q, Yu Y C, Li R, Zhang L W, Liu X L, Zeng D L, Li J Y, Zhou Y H.Brittle Culm 12, a dual-targeting kinesin-4 protein, controls cell-cycle progression and wall properties in rice. Plant J, 2010, 63(2): 312-328. |
| [17] | Li Y H, Qian Q, Zhou Y H, Yan M, Sun L, Zhang M, Fu Z, Wang Y, Han B, Pang X, Chen M, Li J.BRITTLE CULM1, which encodes a COBRA-Like protein, affects the mechanical properties of rice plants. Plant Cell, 2003, 15(9): 2020-2031. |
| [18] | Wu B, Zhang B C, Dai Y, Zhang L, Shang-Guan K K, Peng Y G, Zhou Y H, Zhu Z.Brittle Culm15 encodes a membrane-associated chitinase-like protein required for cellulose biosynthesis in rice. Plant Physiol, 2012, 159(4): 1440-1452. |
| [19] | Ye Y F, Liu B M, Zhao M, Wu K, Cheng W M, Chen X B, Liu Q, Liu Z, Fu X D, Wu Y J.CEF1/OsMYB103L is involved in GA?mediated regulation of secondary wall biosynthesis in rice. Plant Mol Biol, 2015, 89(4-5): 385-401. |
| [20] | Song X J, Kuroha T, Ayano M, Furuta T, Nagai K, Komeda N, Segami S, Miura K, Ogawa D, Kamura T, Suzuki T, Higashiyama T, Yamasaki M, Mori H, Inukai Y, Wu J, Kitano H, Sakakibara H, Jacobsen S E, Ashikari M.Rare allele of a previously unidentified histone H4 acetyltransferase enhances grain weight, yield, and plant biomass in rice.Proc Natl Acad Sci, 2015, 112(1):76-81. |
| [21] | 蔡建秀, 陈伟. 水稻穗上发芽生理生化及颖壳扫描电镜观察. 中国农学通报, 2007, 23(8): 207-211. |
| [21] | Cai J X, Chen W.Study on the physiological biochemistry of pre-harvest sprouting and scanning electron microscopy of glume in rice.Chin Agric Sci Bull, 2007, 23(8): 207-211. (in Chinese with English abstract) |
| [22] | Pogers S O, Bendich A J.Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues.Plant Mol Biol, 1985, 5(2): 569-576. |
| [23] | Brown D M, Goubet F, Wong V W, Goodacre R, Stephens E, Dupree P, Turner S R.Comparison of five xylan synthesis mutants reveals new insight into the mechanisms of xylan synthesis.Plant J, 2007, 52: 1154-1168. |
| [24] | 杨惠杰,杨仁崔,李义珍,姜照伟,郑景生.水稻茎秆性状与抗倒性的关系.福建农业学报,2000, 15(2) :1-7. |
| [24] | Yang H J, Yang R C, Li Y Z, Jiang Z W, Zheng J S.Relationship between culm traits and lodging resistance of rice cultivars.Fujian J Agric Sci, 2000, 15(2): 1-7. (in Chinese with English abstract) |
| [25] | Zhang B C, Zhou Y H.Rice brittleness mutants: A way to open the ‘Black Box’ of monocot cell wall biosynthesis.J Integr Plant Biol, 2011, 53(2): 136-142. |
| [26] | Liu L F, Shangguan K K, Zhang B C, Liu X L, Yan M X, Zhang L J, Shi Y Y, Zhang M, Qian Q, Li J Y, Zhou Y H.Brittle Culm1, a COBRA-like protein, functions in cellulose assembly through binding cellulose microfibrils. PLoS Genet, 2013, 9(8): e1003704. |
| [27] | Aohara T, Kotake T, Kaneko Y, Takatsuji H, Tsumuraya Y, Kawasaki S.RiceBRITTLE CULM 5(BRITTLE NODE) is involved in secondary cell wall formation in the sclerenchyma tissue of nodes.Plant Cell Physiol, 2009, 50(11): 1886-1897. |
| [28] | 吴国超, 桑贤春, 马娇, 朱小燕, 任德勇, 郭爽, 蒋钰东, 杨正林, 凌英华, 何光华. 水稻矮脆突变体dwf1 的特性与基因定位. 植物遗传资源学报, 2014, 15(4): 795-801. |
| [28] | Wu G C, Sang X C, Ma J, Zhu X Y, Ren D Y, Guo S, Jiang Y D, Yang Z L, Ling Y H, He G H.Genetic analysis and fine-mapping of a dwarf and fragile mutant dwf1 in rice.J Plant Genet Resour, 2014, 15(4): 795-801. (in Chinese with English abstract) |
| [29] | 张水金, 郑轶, 朱永生, 杨东, 涂诗航, 周鹏, 郑家团, 黄庭旭. 水稻脆性突变体研究进展. 福建农业学报, 2011, 26(5): 895-898. |
| [29] | Zhang S J, Zheng Y, Zhu Y S, Yang D, Tu S H, Zhou P, Zheng J T, Huang T X.Advance in research on brittleness mutant of rice.Fujian J Agric Sci, 2011, 26(5): 895-898. (in Chinese with English abstract) |
| [30] | 汪海峰, 朱军莉, 刘建新, 吴跃明, 钱前, 腾胜. 饲喂脆茎全株水稻对生长肥育猪生长性能、养分消化和胴体品质的影响. 畜牧兽医学报, 2005, 36(11): 1139-1144. |
| [30] | Wang H F, Zhu J L, Liu J X, Wu Y M, Qian Q, Teng S.Growth performance, nutrient digestibility and carcass quality of growing-finishing pigs fed different levels of whole crop rice brittle culm-1.Acta Veter Zootechn Sin, 2005, 36(11): 1139-1144. (in Chinese with English abstract) |
| [31] | 沈恒胜, 陈君琛, 曾大力, 涂杰峰, 汤葆莎, 滕胜. 低纤维基因突变水稻稻草细胞壁组分动态发育分析. 中国农业科学, 2004, 37(7): 943-947. |
| [31] | Shen H S, Chen J C, Zeng D L, Tu J F, Tang B S, Teng S.Dynamic analysis on composition of cell wall for low-fiber mutation rice.Sci Agric Sin, 2004, 37(7): 943-947. (in Chinese with English abstract) |
| [32] | 吴超, 傅亚萍, 朱丽, 胡国成, 斯华敏, 孙宗修. 转高赖氨酸蛋白基因脆茎水稻的收获指数及秸秆赖氨酸含量的研究. 浙江农业学报, 2008, 20(4): 225-230. |
| [32] | Wu C, Fu Y P, Zhu L, Hu G C, Si H M, Sun Z X.Studies on harvest index and culm lysine content in the transgenic brittle culm rice inserted lysine-rich protein gene.Acta Agric Zhejiang, 2008, 20(4): 225-230. (in Chinese with English abstract) |
/
| 〈 |
|
〉 |