Chinese Journal OF Rice Science >
Genetic Analysis and Gene Mapping of a Pale Green Leaf Mutant HM133 in Rice
Received date: 2015-12-29
Revised date: 2016-03-12
Online published: 2016-11-10
The pale green leaf mutant HM133 was identified from an EMS-induced IR64 mutant bank. The contents of photosynthetic pigments including chlorophyll and carotenoid of HM133 were reduced significantly at 6 weeks and 15 weeks after sowing when compared with IR64.The net photosynthetic rate of HM133 was considerably lower than that of the wild-type IR64 at heading stage while the stomatal conductance was apparently increased. The agronomic traits including plant height, number of filled grain per panicle and seed-setting rate decreased significantly in the mutant compared with the wild-type. In addition, the mutant exhibited a less number of grana, irregular arrangement of thylakoid layer in the chloroplast at the tillering stage. Genetic and mapping analysis showed that the pale green phenotype was controlled by a single recessive gene located in the long arm of chromosome 3 between SSR markers RM143 and RM3684. The interval contains an ORF OsChlD encoding magnesium-chelatase D subunit. Sequence analysis revealed that the mutant allele carried a nucleotide substitution from G to A in the tenth exon of OsChlD, which led to the substitution of glutamic acid for arginine acid. Therefore, it is deduced that OsChlD is the candidate gene controlling the pale green leaf phenotype of HM133.
Key words： Oryza sativa; pale green leaf; gene mapping; magnesium-chelatase; photosynthesis
Yong-feng SHI, Yan HE, Dan GUO, Xiang-guang LV, Qi-na HUANG, Jian-li WU . Genetic Analysis and Gene Mapping of a Pale Green Leaf Mutant HM133 in Rice[J]. Chinese Journal OF Rice Science, 2016 , 30(6) : 603 -610 . DOI: 10.16819/j.1001-7216.2016.6033
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