Chinese Journal OF Rice Science >
Cloning and Characterization of a Small Heat Shock Protein (SHSP) Gene in Rice Plant
Received date: 2016-03-30
Revised date: 2016-04-29
Online published: 2016-11-10
Small heat shock proteins (SHSPs) exist widely in all kinds of creatures and play an important role in both development and stress responses. A gene encoding a SHSP (OsSHSP17.6) was cloned from Oryza sativa and the sequence assembly showed the encoding region of OsSHSP17.6 was 477 bp in length, encoding a polypeptide of 158 aa with a molecular weight of 17.6 kD. Sequence analysis showed that such kind of SHSPs was conserved in different plants and belonged to the cytosolic class I (CI). The transcriptional level of OsSHSP17.6 was significantly up-regulated by heat shock with qRT-PCR. The protein appeared to be accumulated up to higher level under the condition of heat shock in western blotting assays. Taken together, these findings consistently indicated that OsSHPS17.6 could be involved into the response to heat shock, which could contribute to understanding the function of OsSHSP17.6.
Key words： small heat shock protein (SHSP); heat shock; expression pattern
Cong-ying XIANG, Nian-jun CAI, Jing LI, Jian YANG, Jian-ping CHEN, Heng-mu ZHANG . Cloning and Characterization of a Small Heat Shock Protein (SHSP) Gene in Rice Plant[J]. Chinese Journal OF Rice Science, 2016 , 30(6) : 587 -592 . DOI: 10.16819/j.1001-7216.2016.6056
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