Chinese Journal OF Rice Science >
Molecular Characterization of Ustilaginoidea virens T-DNA Insertion Mutant B2510
Received date: 2015-12-31
Revised date: 2016-03-25
Online published: 2016-09-10
To isolate the gene concerned with molecular pathogenic process of Ustilaginoidea virens (U. virens) , T-DNA integration flanking sequence and the mutant genes of a mutant strain B2510 were analyzed. Compared with the wild-type U. virens strain P1, the pathogenicity of the mutant strain B2510 in the field was significantly decreased. The growth rate of B2510 on MM medium was slower than that of P1, but was not significantly different on PSA and TB3 medium which were nutritionally endowed. The mutant strain B2510 did not produce conidiophores in PS broth medium. Genomic Southern bolt analysis confirmed that there were double T-DNA events inserted in genome of mutant strain B2510. The flanking U. virens sequences of T-DNA obtained by TAIL-PCR were adjacent in the wild type and with no sequences lost, and only a few bases of T-DNA were changed. The T-DNA insertion site was in the promoter region of UV8b_1412 and downstream 3'region of UV8b_1386, respectively. RT-PCR analysis confirmed that the expression of both of two genes in B2510 were significantly decreased. The genes which were affected by T-DNA insertion may be associated with pathogenicity and participate in the regulation of pathogenic process of U. virens in rice.
Hui DING, Mi-na YU, Ya-hui WANG, Jun-jie YU, Xiao-le YIN, Hui-wen BO, Xing HUANG, Yong-feng LIU . Molecular Characterization of Ustilaginoidea virens T-DNA Insertion Mutant B2510[J]. Chinese Journal OF Rice Science, 2016 , 30(5) : 541 -551 . DOI: 10.16819/j.1001-7216.2016.5198
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