Chinese Journal OF Rice Science >
Genetic Analysis and Gene Mapping of a Dwarf and High-tillering Mutant mz3 in Rice (Oryza sativa L.)
Received date: 2016-03-04
Revised date: 2016-05-02
Online published: 2016-09-10
A dwarf and high-tillering mutant, temporarily termed as mz3 was obtained from Zhonghua 11, a japonica rice variety by EMS mutagenesis. Genetic analysis indicated that the mutant phenotype was controlled by a recessive gene. An F2 population was developed by crossing mz3 with Nanjing 11, an indica rice variety for mapping of the mutant gene, which was ultimately restricted within a physical distance of about 747 kb between two SSR markers RM19353 and RM510 on the long arm of rice chromosome 6. Gene prediction revealed that D3, a gene conferring rice plant height and tiller number was located in this region. Sequence analysis identified a single G to A nuclear acid substitution at the position 636 from the ATG start codon, forming a premature translational termination codon. Sequencing of 10 randomly selected recessive plants from mz3/Nanjing 11 mapping population confirmed the same mutation site carried by the 10 individuals. Subcelluar location indicated that D3 protein of the mutant was located in the nuclei as wild type. BiFC analysis showed that D3 protein of the mutant could not interact with D14 protein for lacking of essential amino acids that bind with D14, blocking signal transduction of strigolactones. So we speculated that the mutant phenotype of mz3 was probably caused by mutation in D3 gene.
Key words： rice; dwarf and high-tillering; D3; gene mapping
Xiao-mei SU, Yu-xing FANG, Yu-long LIU, Feng LIU, Suo-bing ZHANG, Yun-hui ZHANG, Yi-qun BAO . Genetic Analysis and Gene Mapping of a Dwarf and High-tillering Mutant mz3 in Rice (Oryza sativa L.)[J]. Chinese Journal OF Rice Science, 2016 , 30(5) : 479 -486 . DOI: 10.16819/j.1001-7216.2016.6037
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