Chinese Journal OF Rice Science >
Global Genome Expression Analysis of Root Genes under Drought Stress in Weedy Rice and Up-land Rice
Received date: 2015-12-09
Revised date: 2016-01-23
Online published: 2016-09-10
The expression changes of root genes in drought-resistance weedy rice HEB07-2 (Oryza sativa f. spontanea) and up-land rice IAPAR9 (Oryza sativa) were analyzed under polyethylene glycol(PEG)-simulated with drought stress condition with Affymetrix GeneChip rice genome array. The results indicated that the extent and direction of transcriptome response of HEB07-2 and IAPAR9 to drought differed greatly. For HEB07-2, among 6878 expressed genes, 4693 were up-regulated and 2185 were down-regulated under drought condition. For IAPAR9, among 2923 expressed genes,983 were up-regulated and 1940 were down-regulated. Analysis of differentially expressed genes in HEB07-2 and IAPAR9 showed that the weedy rice HEB07-2 had a higher changing fold than the up-land rice IAPAR9. Gene ontology analysis revealed that genes of HEB07-2 in potassium ion transporting(GO: 0006813), secondary metabolite(GO: 0019748), cell growth(GO: 0016049), glucose metabolism(GO:0006006), transmembrane ion transporter activity(GO:0015075), ferroheme coalition(GO:0020037), oxidordeuctase activity(GO:0016491)were significantly up-regulated in comparing with IAPAR9. These were consistent with the physiological and phenotypic data.
Key words： weedy rice; root; gene expression profiling; drought resistance
Guo-hua DING, Jian SUN, Guang YANG, Feng-ming ZHANG, Liang-ming BAI, Shi-chen SUN, Shu-kun JIANG, Tong-tong WANG, Hong-liang ZHENG, Tian-shu XIA, Xi-hong SHEN, Dian-rong MA, Wen-fu CHEN . Global Genome Expression Analysis of Root Genes under Drought Stress in Weedy Rice and Up-land Rice[J]. Chinese Journal OF Rice Science, 2016 , 30(5) : 458 -468 . DOI: 10.16819/j.1001-7216.2016.5180
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