Chinese Journal OF Rice Science >
Roles of Transcription Factor Gene OsPHR3 on the Utilization of Phosphate in Rice
Received date: 2016-01-22
Revised date: 2016-02-04
Online published: 2016-07-10
Phosphorus, an essential macronutrient element for plant growth and development, is broadly participating in various plant life activities. However, low availability of soil phosphate is a major constraint for crop production in many agricultural systems. OsPHR3（LOC_Os02g04640）is a homolog of OsPHR2, the central regulator of Pisignaling in rice. Both OsPHR2and OsPHR3 belong to the MYBCC family members and have functional redundancy in part. In this study, OsPHR3 knockout and overexpression transgenic plants were obtained. The hydroponic culture, 32Pi isotopic and pot experiments were used to demonstrate the role of OsPHR3 in rice phosphate untilization. First, hydroponic experiments showed that overexpression of OsPHR3increased the phosphorus content of the rice, although the knockout lines had no significant difference with wild type plants. Second, 32Pi isotopic experiments revealed that overexpression of the gene promoted the absorption and distribution of phosphate, while the absorption rate in knockout lines decreased in comparison with wild type plants under Pideficient condition. Third, pot experiments demonstrated that overexpression of OsPHR3 enhanced the effective tillers and phosphorus content in seed. However, the panicle length in knockout lines is shorter than in wild type plants. In conclusion, OsPHR3 is a potential transcription factor for improving P efficiency in rice through molecular breeding.
ZHANG Liang, SUN Wenxian, SUN Yafei, PEI Wenxia, LUO Wenzhen, SUN Rui, ZHANG Zhantian, XU Guohua, SUN Shubin* . Roles of Transcription Factor Gene OsPHR3 on the Utilization of Phosphate in Rice[J]. Chinese Journal OF Rice Science, 2016 , 30(4) : 397 -405 . DOI: 10.16819/j.1001-7216.2016.6011
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