Chinese Journal OF Rice Science >
Characterization and Gene Mapping of Lesion Mimic Mutant wy3 in Rice
# These authors contributed equally to this work;
Received date: 2015-11-30
Revised date: 2015-12-24
Online published: 2016-05-10
A lesion mimic mutant wy3 was obtained from the progeny of a japonica variety Wuyujing 3 by natural mutation. The lesions were first observed on the leaves at the seedling stage, then spread gradually to the whole leaf at the initial tillering stage. Compared with the wild type(WT), the plant height, the number of effective tiller, panicle length, grain number per panicle and seed setting rate of wy3 were reduced significantly. The shading assay showed that the lesions on leaves of wy3 were induced by natural light. Compared with the wild type, the photosynthetic pigment and the net photosynthetic rate of wy3 were significantly reduced, but the SOD activity, POD activity, CAT activity and MDA content of wy3 were significantly higher than those of WT. Trypan blue staining experiments showed that there were a large number of dead cells in the mutant lesion. Genetic analysis suggested that the phenotype of wy3 was controlled by a single recessive nuclear locus, and the target gene was mapped between markers W2-17 and W2-18 with the physical distance of 28 kb on chromosome 2. Sequence analysis revealed that the mutated gene in wy3 had a single nucleotide deletion at the 375th in CDS of LOC_Os02g02000, resulting in a premature termination of translation of the target gene, which is an new allele of OsHPL3.
Key words： rice; lesion mimic mutant; gene mapping
Hong-Gen ZHANG, Mao-yu WANG, Li-jia ZHANG, Ya HU, Jia-qi MA, Yi-fan ZHANG, Shu-zhu TANG, Guo-hua LIANG, Ming-hong GU . Characterization and Gene Mapping of Lesion Mimic Mutant wy3 in Rice[J]. Chinese Journal OF Rice Science, 2016 , 30(3) : 239 -246 . DOI: 10.16819/j.1001-7216.2016.5176
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