Chinese Journal OF Rice Science >
Phenotypical Analysis and Fine Mapping of a Semi-dominant Dwarfism Gene Si-dd1 in Rice
Received date: 2015-07-13
Revised date: 2015-10-18
Online published: 2016-03-10
A semi-dominant dwarf rice mutant (termed as Si-dd1) was obtained from japonica rice variety Nipponbare by tissue culture mutagenesis. Morphological analysis showed that Si-dd1(AA) and Si-dd1(Aa) exhibited shorter plant height, decreased seed-setting rate, delayed heading, increased number of primary rachis branch and secondary rachis branch compared to the wild type (WT,Nipponbare). Physiological test results showed Si-dd1 had similar response to 24-Epibrassinosteroid to the WT, while it was insensitive to Gibberellin at 10-4μmol/L. Moreover, Western blot results also confirmed GID2 expression level in Si-dd1 differed greatly from WT. The cytological analysis showed that Si-dd1 had smaller stomas in the leaf midrib, increased mesophyll cells and stem vascular bundles compared to WT. Genetic analysis and map-based clone showed that the Si-dd1 is controlled by a single semi-dominant gene, and locates in a 244 kb region on chromosome 6.
Key words： rice; semi-dominant; dwarf; genetic analysis; map-based clone
Yong-tao CUI, Li-wen WU, Shi-kai HU, De-yong REN, Chang-wei GE, Wei-jun YE, Guo-jun DONG, Long-biao GUO, Xing-ming HU . Phenotypical Analysis and Fine Mapping of a Semi-dominant Dwarfism Gene Si-dd1 in Rice[J]. Chinese Journal OF Rice Science, 2016 , 30(2) : 152 -160 . DOI: 10.16819/j.1001-7216.2016.5113
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