Chinese Journal OF Rice Science >
Overexpressing Analysis of the Flowering Time Gene OsDTH10 in Rice
Received date: 2015-09-21
Revised date: 2015-12-17
Online published: 2016-03-10
Flowering time (heading date) is an important agronomic trait of crops, and it is closely linked with the yield of crops. To reveal the function of rice flowering time gene OsDTH10 in rice, we constructed an overexpressing vector of OsDTH10 and analyzed the function of OsDTH10 by the reverse genetics. The results showed that the OsDTH10-overexpressing (OsDTH10-OV) transgenic plants displayed a late heading phenotype, suggesting that the overexpression of OsDTH10delayed the heading-date of rice. RT-PCR expression analysis showed that the expression level of OsDTH10was increased obviously in OsDTH10-OV lines with phenotype compared with the transgenic lines without phenotype and wild type plants, indicating that late heading phenotype in OsDTH10-OV lines was caused by the overexpression of OsDTH10. The tissue-specific expression showed that OsDTH10 was expressed in different organs, with the higher expression levels in stem and leaf sheath. Also, we further analyzed the expression of OsDTH10 in leaves at various leaf-ages, and the result showed that OsDTH10 exhibited higher expression in unexpanded flag leaf and the second leaf from the top than these in the third and the fourth leaf from the top. In addition, we also analyzed the expression of OsDTH10 in different photoperiods, and the result indicated that OsDTH10displayed a higher expression during the day time (light stage) and a lower expression at night (dark stage) whatever short-day and long-day conditions, suggesting that OsDTH10 might be involved in the photoperiodic pathways to regulate the heading-date in rice.
Key words： rice; OsDTH10; overexpression; expression analysis
Ying-ying LI, Wei XIONG, Ting SONG, Zhen-hua CAO, Wei-jiang LUAN . Overexpressing Analysis of the Flowering Time Gene OsDTH10 in Rice[J]. Chinese Journal OF Rice Science, 2016 , 30(2) : 121 -126 . DOI: 10.16819/j.1001-7216.2016.5140
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