Chinese Journal OF Rice Science >
Expression Profiles of Rice WRKY Transcription Factor Gene Family Responsive to Exogenous Nitric Oxide Application
Received date: 2015-09-16
Revised date: 2015-11-30
Online published: 2016-03-10
Signal molecule nitric oxide (NO) and WRKY transcription factors are involved in many physiological processes, such as defense against stresses, development and metabolism in plants. In the present study, we investigated the expression patterns of WRKY transcription factor gene family in rice seedlings at 1, 6 and 12 after exogenous NO treatments using Agilent rice cDNA oligo microarray. Totally, 32 differently expressed WRKY genes, whose expression levels increased or decreased by at least two-fold at one time point compared with the control (T0), were identified. These WRKY genes were mainly distributed over Groups Ⅰ and Ⅱ, among which 75% of Ⅱa and 45.6% of Ⅱd subgroup members were differently expressed upon the NO treatments. Moreover, 15 identified NO-responsive WRKY genes, whose expression level changed by more than two-fold at least two time points among the three time points compared with the control, exhibited an earlier (1 h) response to the NO treatments, and most of them (64.2%) were continually up-regulated. Prediction of gene function revealed that the NO-responsive WRKY genes were mainly involved in cellular process, metabolic process and response to stimulus of biological process, and transcription regulator activity and binding of molecular function. The analysis of metabolic pathways showed that WRKY24 was involved in plant-pathogen interaction pathway. The results of microarray hybridization were largely consistent with those of quantitative real-time PCR, verifying the validity of microarray hybridization. These findings suggest that NO signaling might be involved in the regulatory functions of WRKY transcription factors, and provide a basis for further functional research for these differentially expressed WRKY genes.
Jiao MENG, Hai-hua WANG, Jian-hua XIANG, Dan JIANG, Xi-xu PENG, Huan-huan HE . Expression Profiles of Rice WRKY Transcription Factor Gene Family Responsive to Exogenous Nitric Oxide Application[J]. Chinese Journal OF Rice Science, 2016 , 30(2) : 111 -120 . DOI: 10.16819/j.1001-7216.2016.5138
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