Chinese Journal OF Rice Science >
Cloning and Expression of the Gene OC-XII Conferring Resistance to Aphelenchoides besseyi in Rice
*Corresponding author：E-mail:danleili@126.com
Received date: 2014-11-11
Revised date: 2015-05-18
Online published: 2015-11-10
The previous resistance verification indicated that Dabaigu (Oryza sativa ssp. indica) resists the infection of Aphelenchoides bessey. To reveal the interaction between the resistance and the pathogenesis-related protein Cystatin, the gene OC-XII was cloned, the docking between OC-XII and A. besseyi Cathepsin B was done, and the expression of OC-XII was tested by quantitative real-time PCR. Results showed that OC-XII bound with Cathepsin B specifily. The expression level of OC-XII was upregulated from 12 hours after infection to 2 days after infection, and downregulated 3 days after infection. These results indicated that OC-XII was involved in the resistance to A. besseyi. The gene has the potential for biological control of plant-parasitic nematodes, and it is necessary to conduct in-depth research.
Key words： cystatin; inhibitor; Aphelenchoides besseyi; Cathepsin B
Feng WANG, Qiao-li CHEN, Dan-lei LI, Ya-ming LING, Bo-wen WANG, Rui-zhi ZHANG, Yan-yan JIA, Yong-hang LI, Yu-ying PEI, Xin-ming LU . Cloning and Expression of the Gene OC-XII Conferring Resistance to Aphelenchoides besseyi in Rice[J]. Chinese Journal OF Rice Science, 2015 , 29(6) : 658 -664 . DOI: 10.3969/j.issn.1001G7216.2015.06.013
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