Chinese Journal OF Rice Science >
Expression of OsWRKY7 in Rice
*Corresponding author：E-mail:jpchen2001@126.com，yangchengqi@163.com
Received date: 2015-04-22
Revised date: 2015-07-18
Online published: 2015-11-10
WRKY proteins are important transcription factors in plants. They are involved in various plant developmental processes, as well as in coping with diverse biotic and abiotic stresses. In this study,we cloned the coding sequence (CDS) and upstream promoter of OsWRKY7 from Nipponbare to analyze its expression patterns. We first analyzed the relative expression level of OsWRKY7 in different tissues by quantitative real-time PCR(qRT-PCR), and the result showed that OsWRKY7 is mainly expressed in leaves, with a higher expression level in flag leaves than that in seedling leaves. We then constructed the pOsWRKY7-GUS expression vector by fusing the putative promoter with GUS reporter gene and transformed the vector into Nipponbare. Subsequent GUS staining showed that OsWRKY7 promoter had activity in primary root tip, leaf blade and glume. Consistent to the qRT-PCR result, massive GUS spots were stained on the entire leaf blade. We also characterized the inducibility of the pOsWRKY7-GUS transgenic plants to pathogen infection and hormone treatment. The results showed that the GUS activities in both leaf and root are up-regulated after inoculation with rice bacterial blight pathogen [Xanthomonas oryzae pv. Oryzae (Xoo)] strain P10, as well as after exogenous application of cytokinin and auxin, while salicylic acid treatment represses GUS activity in both leaf and root. Finally, we fused the CDS of OsWRKY7 with the green fluorescent protein and the GAL4 DNA binding domain, respectively to analyze its subcellular localization in rice and transcriptional activity in yeast. The results showed that OsWRKY7 was localized exclusively to the nucleus of rice stem protoplasts, and has transcriptional self-activation activity in yeast. All these data suggested that OsWRKY7 might act as a transcriptional activator in bacterial blight defense and diverse hormone signal transduction pathways.
Ru LI, Jie ZHOU, Dong-yue LI, Xu-ming WANG, Yong YANG, Chu-lang YU, Ye CHENG, Cheng-qi YAN, Jian-ping CHEN . Expression of OsWRKY7 in Rice[J]. Chinese Journal OF Rice Science, 2015 , 29(6) : 559 -570 . DOI: 10.3969/j.issn.1001G7216.2015.06.001
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