Chinese Journal OF Rice Science >
Analysis of Expression Characteristics of Isoamylase and the Correlation with Starch Content During Grain Filling in Rice
*Corresponding author：E-mailzxjin326@hotmail.com
Received date: 2015-01-21
Revised date: 2015-04-10
Online published: 2015-09-10
By using five japonica rice varieties with different amylose and amylopectin contents in endosperm as experimental material, we analyzed the expression levels of ISAs and the activity of isomylase, and their correlation with starch content during grain filling. It turned out that the isomylase activity, the expression levels of ISA1 and ISA3 of tested varieties followed a unimodal curve with peak value appearing during the mid-filling stage except sticky rice, while for the sticky rice, the expression level showed a straight-line growth during grain filling with obvious higher expression in late stage. The expression level of ISA2 was down-regulated ten days after heading. In principle, the expression levels of ISA1 and ISA3 were significantly positively correlated with the enzyme activity, and they both synchronously changed with the content of amylopectin. During grain filling, the expression levels of ISA1 and ISA3 were absolutely higher than that of ISA2, indicating that they were dominated genes expressed during grain filling.
Li-nan ZHU, Hai-ying LIU, Lu-lu SUN, Tao SUN, Xue-dong GUO, Fang-xu ZHU, Zhong-chen ZHANG, Zheng-xun JIN . Analysis of Expression Characteristics of Isoamylase and the Correlation with Starch Content During Grain Filling in Rice[J]. Chinese Journal OF Rice Science, 2015 , 29(5) : 528 -534 . DOI: 10.3969/j.issn.1001G7216.2015.05.010
| [1] | James M G, Robertson D S, Myers A M.Characterization of the maize gene sugary1, a determinant of starch composition in kernels.Plant Cell, 1995, 7(4): 417-429. |
| [2] | Zeeman S C, Umemoto T, Lue W L, et al.A mutant of Arabidopsis lacking a chloroplastic isoamylase accumulates both starch and phytoglycogen.Plant Cell, 1998, 10(10): 1699-1712. |
| [3] | Utsumi Y, Utsumi C, Sawada T, et al.Functional diversity of isoamylase oligomers: The ISA1 homo-oligomer is essential for amylopectin biosynthesis in rice endosperm.Plant Physiol, 2011,156(1): 61-77. |
| [4] | Nakamura Y, Umemoto T, Takahata Y, et al.Changes in structure of starch and enzyme activities affected by sugary mutations in developing rice endosperm: Possible role of starch debranching enzyme (R enzyme) in amylopectin biosynthesis.Plant Physiol, 1996, 97(1): 491-498. |
| [5] | Nakamura Y.Towards a better understanding of the metabolic system for amylopectin biosynthesis in plants: Rice endosperm as a model tissue.Plant Cell Physiol, 2002, 43(7): 718-725. |
| [6] | Genschel U, Abel G, Lörz H, et al.The sugary-type isoamylase in wheat: Tissue distribution and subcellular localisation.Planta, 2002, 214(5): 813-820. |
| [7] | Kubo A, Fujita N, Harada K, et al.The starch debranching enzymes isoamylase and pulluanase are both involved in amylopectin biosynthesis in rice endosperm.Plant Physiol, 1999, 121(2): 399-409. |
| [8] | 曲莹,金正勋,刘海英,等. 粳稻杂种后代胚乳可溶性淀粉合成酶及同工型基因表达分析. 中国水稻科学,2014,28(1): 23-31. |
| [9] | 何照范. 粮油籽粒品种及其分析技术. 北京: 中国农业出版社, 1985:137-139. |
| [10] | Liang J S, Zhang J H, Cao X X.Grain sink strength may be related to the poor grain filling of indica-japonica rice (Oryza sativa L.) hybrids.Physiol Plant, 2001, 112(4): 470-477. |
| [11] | 吕冰, 郭志刚, 梁建生. 水稻胚乳中淀粉合成相关酶活性的变化对支链淀粉精细结构的影响. 中国科学C辑: 生命科学, 2008, 38(8): 766-773. |
| [12] | Jain M, Nijhawan A, Tyagi A K, et al.Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR.Biochem Biophy Res Commun, 2006, 345(2): 646-651. |
| [13] | 王云飞, 赵法茂, 李天骄, 等. 小麦籽粒灌浆过程中淀粉去分支酶的类型、活性及其纯化. 作物学报, 2007, 33(11): 1840-1844. |
| [14] | 李钱峰, 张桂云, 于恒秀, 等. 水稻异淀粉酶基因ISA1及其启动子的表达特性分析. 中国水稻科学, 2009, 23(1): 12-18. |
| [15] | Ohdan T, Francisco P B, Sawada T, et al.Expression profiling of genes involved in starch synthesis in sink and source organs of rice.J Exp Bot, 2005, 56(422): 3229-3244. |
| [16] | Duan M J, Sun S M.Profiling the expression of genes controlling rice grain quality.Plant Mol Biol, 2005, 59: 165-178. |
| [17] | Kubo A, Rahman S, Utsumi Y, et al.Complementation of sugary-1 phenotype in rice endosperm with the wheat isoamy- lase1 gene supports a direct role forisoamylase1 in amylopectin biosynthesis.Plant Physiol, 2005, 137(1): 43-56. |
| [18] | Yun M S, Umemoto T, Kawagoe Y.Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis.Plant Cell Physiol, 2011, 52(6): 1068-1082. |
| [19] | Streb S, Eicke S, Zeeman S C.The simultaneous abolition of three starch hydrolases blocks transient starch breakdown in Arabidopsis.Biol Chem,2012,287(50): 41745-41756. |
| [20] | 钟连进, 董虎, 蔡小波, 等. 控制水稻胚乳淀粉合成代谢若干关键酶基因对花后高温的响应表达. 应用生态学报, 2012, 23(3): 745-750. |
| [21] | 钟连进, 程方民, 张国平, 等. 灌浆结实期不同温度下早籼稻米淀粉链长分布与结构特征差异分析. 中国农业科学, 2005, 38(2): 272-276. |
/
| 〈 |
|
〉 |