Chinese Journal OF Rice Science >
Characterization and Gene Mapping of an Open Hull Male Sterile Mutant ohms1 Caused by Alternative Splicing in Rice
*Corresponding author：E-mailcaoliyong@cass.cn;shcheng@mail.hz.zj.cn
Received date: 2015-02-04
Revised date: 2015-05-06
Online published: 2015-09-10
A rice floral organ development mutant was obtained from the 60Co-γ-treated indica restorer line Zhonghui 8015 and termed as open hull and male sterile 1 (ohms1). The mutant was characterized as open hull and lemma- and palea-like structure between anthers and stigma that made the spikelet of the mutant showed three “triodia-like ” flower glumes. The mutant was self-sterile but 60%-70% pollen were fertile. Genetic analysis and gene mapping showed ohms1 was controlled by a single ressessive gene and the mutant gene was fine mapped to a region of 42 kb on the short arm of chromosome 3 between markers KY2 and KY29. Sequence analysis of the four open reading frames(ORFs) in this region revealed that LOC_Os03g11614 was probably corresponding to ohms1, which encoded a MADS-box gene allelic with OsMADS1, and had a single nucleotide transformation (A to G) at the bottom of the fifth intron. Enzyme digestion and cDNA sequencing furtherly indicated that the variable splicing was responsible for the delection of the sixth exon of ohms1,but no structure changes in MADS domain or amino acid frameshift occurred. Additionally,real-time fluorescence quantitative PCR analysis showed that the expression level of OHMS1 decreased significantly and the expression level of rice flowering factors and floral glume development-related genes changed significantly. All those results demonstrated that OHMS1 may play an important role in rice floral organ development,particularly in floral glume development and floret primordium differentiation.
Key words： rice; open hull male sterile; gene mapping; alternative splicing
Lian-ping SUN, Ying-xin ZHANG, Pei-pei ZHANG, Zheng-fu YANG, Xiao-deng ZHAN, Xi-hong SHEN, Zhen-hua ZHANG, Xia HU, Dan-dan XUAN, Wei-xun WU, Li-yong CAO, Shi-hua CHENG . Characterization and Gene Mapping of an Open Hull Male Sterile Mutant ohms1 Caused by Alternative Splicing in Rice[J]. Chinese Journal OF Rice Science, 2015 , 29(5) : 457 -466 . DOI: 10.3969/j.issn.1001G7216.2015.05.002
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