Chinese Journal OF Rice Science >
Changes in Growth and Development and Main Enzyme Activities in Midgut of Cnaphalocrocis medinalis Intermittently Treated With Low Amount of Bt Rice Leaves over Generations
Received date: 2014-09-11
Revised date: 2015-03-12
Online published: 2015-07-10
The developmental indices and main enzyme activities in midgut of Cnaphalocrocis medinalis treated with low amount of Bt rice leaves of T2A-1 (harboring cry2A gene) for three generations (5 h for every generation) were investigated to understand the response of C. medinalis to Bt rice. Results showed that developmental duration of 3rd-5th instar C. medinalis fed on Bt rice leaves in the 1st generation were prolonged and the emergence rates of C. medinalis sharply decreased compared with the untreated C. medinalis. However, these two indices returned to the level of the untreated C. medinalis with the increasing generations. The mortalities of the 3rd-5th instar C. medinalis larvae and the weight of female pupa lowered, and the weight of male pupa did not differ from that of untreated C. medinalis. Furthermore, the mortality of the 3rd-5th instar C. medinalis larvae and weight of male and female pupa did not return to or remain the levels of the untreated C. medinalis as the treated generations increased. These indices for the treated 3rd generation C. medinalis were lower than those of untreated C. medinalis. The total proteinase activities in C. medinalis larvae midgut juice fed on Bt rice leaves were lower than those in untreated C. medinalis, but the total proteinase activities followed an increasing trend with the increasing generations, and the similar trend was observed in the chymotrypsin-like enzyme activity of C. medinalis. While the activities of trypsin in the treated C. medinalis were higher than that in untreated C. medinalis and increased as treated generations increased, and the similar trend was observed in the aminopeptidase activity of C. medinalis. These results indicated that C. medinalis has potential adaptive ability under exposure to low amount of Cry2A protein.
Zhi-hong WU, Ya-jun YANG, Hong-xing XU, Xu-song ZHENG, Jun-ce TIAN, Yan-hui LU, Zhong-xian LV . Changes in Growth and Development and Main Enzyme Activities in Midgut of Cnaphalocrocis medinalis Intermittently Treated With Low Amount of Bt Rice Leaves over Generations[J]. Chinese Journal OF Rice Science, 2015 , 29(4) : 417 -423 . DOI: 10．3969/j．issn．1001G7216．2015．04．011
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