Chinese Journal OF Rice Science >
Effect of RBSDV Infection on Transcriptional Expression of Abscisic Acid Metabolism Related Genes in Rice
Received date: 2014-12-01
Revised date: 2015-01-29
Online published: 2015-05-10
To investigate the effect of virus infection on abscisic acid (ABA) content in rice, two rice varieties, Nipponbare and Huaidao 5, were inoculated with rice black streaked dwarf virus (RBSDV) artificially via small brown planthoppers, and the ABA content was measured by ELISA method when the plants showed the obvious viral disease symptoms. The results showed that the ABA content was increased in virus infected rice. The ABA content in virus infected Nipponbare was 111.04 ng/g compared to 60.86 ng/g in the control. In Huaidao 5, it was 102.60 ng/g in the virus infected plants and 70.61 ng/g in the control. To reveal how the virus regulates the ABA content, the relative expression levels of genes involved in ABA biosynthesis (OsZEP, OsNCED1, OsNCED2, OsNCED3, OsNCED4 and OsNCED5) and catabolism (OsABA8ox1, OsABA8ox2 and OsABA8ox3) were analyzed in Nipponbare at 8 d, 12 d, 16 d and 60 d after virus infection. The qRT-PCR results showed that the expression levels of the genes involved in ABA biosynthesis and catabolism in the plants than at 8 days after virus infection were higher than those in the control. The expression levels of OsNCED4 and OsNCED5 increased continuously with infection duration. At 60 d, the expression levels of OsNCED3, OsNCED4 and OsNCED5 in RBSDV infected plants were 3.97-, 7.66- and 2.99-fold higher than those in the control, and the expression levels of OsZEP, OsABA8ox1 and OsABA8ox2 were decreased with time extension. The data suggested that RBSDV infection affected the ABA biosynthesis and catabolism metabolism pathway, resulting in the increase of ABA content.
Key words： RBSDV; rice; ABA; biosynthesis; catabolism; gene expression
Hai-ping NI, Qiu-fang XU, Ying LAN, Qing-qing CHEN, Jin-feng ZHANG, Yi-jun ZHOU . Effect of RBSDV Infection on Transcriptional Expression of Abscisic Acid Metabolism Related Genes in Rice[J]. Chinese Journal OF Rice Science, 2015 , 29(3) : 319 -326 . DOI: 10．3969/j．issn．1001G7216．2015．03．012
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