Chinese Journal OF Rice Science >
Genetic Analysis and Gene Mapping of a Yellow-green Leaf Mutant w390 in Rice (Oryza sativa L.)
Received date: 2014-11-27
Revised date: 2014-12-24
Online published: 2015-05-10
A yellow-green leaf mutant, designated as w390, was isolated from the progeny of 60Co-γ-treated indica cultivar R8015. The mutant exhibited a stable yellow-green leaf phenotype during the whole life cycle. The chlorophyll b was not detected in w390, and the chlorophyll a and carotenoids contents of w390 were reduced by 50.6% and 44.8%, compared with the wild-type. The plant height, the number of panicle per plant, the number of spikelets per panicle, the number of grains per panicle of w390 were reduced by 12.0%, 22.3%,18.5% and 27.6%, respectively. The transmission electron microscope assay demonstrated that the number of thylakoids decreased and granas were poorly stacked in the mutant, resulting in underdevelopment of chloroplasts. Genetic analysis showed that the yellow-green leaf phenotype was controlled by a single recessive gene. Using an F2 mapping population derived from the cross of w390 mutant and Nipponbare, the gene was delimitated to a region of 71.8-kb on the long arm of chromosome 10, which contained 15 ORFs. Sequence analysis revealed that the mutant carried two nucleotide substitutions in the eighth exon of OsCAO1 gene, which led to the substitution of leucine and glycine for histidine and glutamic acid, respectively. This implied that W390 might be a novel allele of OsCAO1 gene.
Key words： Oryza sativa L.; mutant; yellow-green leaf; gene mapping
Qing DONG, Ying-xin ZHANG, Zhen-hua ZHANG, Quan ZHOU, Ya-zhi QIN, Hong WANG, Shi-hua CHENG, Li-yong CAO, Xi-hong SHEN . Genetic Analysis and Gene Mapping of a Yellow-green Leaf Mutant w390 in Rice (Oryza sativa L.)[J]. Chinese Journal OF Rice Science, 2015 , 29(3) : 241 -249 . DOI: 10．3969/j．issn．1001G7216．2015．03．003
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