Chinese Journal OF Rice Science >
Dissection and QTL Mapping of Low-phosphorus Tolerance Using Selected Introgression Lines in Rice
Received date: 2014-04-28
Revised date: 2014-05-08
Online published: 2015-01-10
Two BC2F4 backcrossing populations were generated by using the two widely used elite indica restorers in China, Shuhui 527 and Minghui 86 as recurrent parents, the indica variety Yetuozai as donor. The grain yield and its related traits were evaluated under normal fertilizer application level in normal soil (as control) and in poor soil, low phosphorus stress conditions in poor soil in Langfang, Hebei Province. Meanwhile, the introgressed lines (ILs) of two populations were investigated at seedling stage using nutrient solution in the glasshouse in Beijing. A total of 49 main-effect QTLs underlying yield related traits were identified, the contribution of phenotypic variations ranged from 6.7% to 16.5%. Of which, 25 (51.0%) QTLs had favorable alleles from donor parent. A total of 48 main-effect QTLs were identified for yield related traits, the contribution of phenotypic variations ranged from 7.7% to 16.6%. Of which, 21(43.8%) QTLs had favorable alleles from donor parent. About 79.6% of the QTLs can be detected repeatedly under two or more treatments, especially those QTLs associated with grain number per panicle, spikelet fertility and thousand grain weight which displayed consistent phenotypic effects. Among all detected QTLs, eight could be simultaneously identified across two environments. RM511(Bin.12.4) on chromosome 12 showed the most strong pleiotropism, simultaneously controlling filled grain weight, total grain weight per panicle, root length, dry weight of root, dry weight of shoot and total dry weight.
Jie REN, Xiu-qin ZHAO, Zai-song DING, Chao XIANG, Jing ZHANG, Chao WANG, Jun-wei ZHANG, Augustino JOSEPH Charles, Qiang ZHANG, Yun-long PANG, Yong-ming GAO, Ying-yao SHI . Dissection and QTL Mapping of Low-phosphorus Tolerance Using Selected Introgression Lines in Rice[J]. Chinese Journal OF Rice Science, 2015 , 29(1) : 1 -13 . DOI: 10.3969/j.issn.1001-7216.2015.01.001
| [1] | Vinod K K, Heuer Sigrid.Approaches towards nitrogen- and phosphorus-efficient rice.AoB Plants, 2012, doi:10.1093/aobpla/pls028. |
| [2] | Carriger S, Vallee D.More crop per drop.Rice Today, 2007, 6: 10-13. |
| [3] | 吴平. 植物磷高效吸收利用的分子机制研究及其应用. 中国科技成果, 2013 (9): 20-20. |
| [4] | 穆平, 黄超, 李君霞, 等.低磷胁迫下水稻产量性状变化及其定位. 作物学报, 2008, 34(7):1137-1142 |
| [5] | Loudet O, Chaillou S, Merigout P, et al.Quantitative trait loci analysis of nitrogen use efficiency in Arabidopsis.Plant Physiol, 2003, 131(1): 345-358. |
| [6] | 杜娟, 曾亚文, 杨树明, 等.水稻磷高效重组自交系群体的筛选鉴定. 生态环境, 2008, 17(3): 1151-1156. |
| [7] | Tanksley S D, Nelson J C.Advanced backcross QTL analysis: A method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines.Theor Appl Genet, 1996, 92(2): 191-203. |
| [8] | Li Z K, Fu B Y, Gao Y M, et al.Genome-wide introgression lines and their use in genetic and molecular dissection of complex phenotypes in rice (Oryza sativa L.).Plant Mol Biol, 2005, 59(1): 33-52. |
| [9] | 罗利军. 水稻等基因导入系构建与分子技术育种.分子植物育种, 2005, 3(5): 609-612. |
| [10] | Chen M Y, Ali J, Fu B Y, et al.Detection of drought-related loci in rice at reproductive stage using selected introgressed lines.Agric Sci China, 2011, 10(1): 1-8. |
| [11] | Moncada P, Martinez C P, Borrero J, et al.Quantitative trait loci for yield and yield components in an Oryza sativa-Oryza rufipogon BC2F2 population evaluated in an upland environment.Theorl Appl Genet, 2001, 102(1): 41-52. |
| [12] | GRAMENE. GRAMENE Database.[2006-4-5] |
| [13] | 郑天清, 徐建龙, 傅彬英, 等.遗传搭车与方差分析在水稻定向选择群体的抗旱性位点分析中的初步应用.作物学报, 2007, 33(5): 799-804. |
| [14] | SAS Institute Inc.ed., 2008, SAS/STAT V9.2 user’s guide, SAS Institute Inc, Cary, NC, USA: 2430-2611. |
| [15] | 高方远, 陆贤军, 康海岐, 等.水稻耐低磷种质的苗期筛选与鉴定.作物学报, 2006, 32(8): 1151-1155. |
| [16] | 张帆,郝宪彬,高用明, 等.利用籼稻资源中的“隐蔽有利基因”提高粳稻苗期耐冷性.作物学报, 2007, 33(10): 1618-1624. |
| [17] | He Y X, Zheng T Q, Hao X B, et al.Yield performances of japonica introgression lines selected for drought tolerance in a BC breeding programme.Plant Breeding, 2010, 129(2): 167-175. |
| [18] | Meng L J, Lin X Y, Wang J M, et al.Simultaneous improvement of cold tolerance and yield of temperate japonica rice (Oryza sativa L.) by introgression breeding.Plant Breeding, 2013, 132(6): 604-612. |
| [19] | 徐建龙,高用明,傅彬英,等.回交导入后代水稻种质有利基因的鉴定与筛选研究. 分子植物育种, 2005, 3(5): 619-628. |
| [20] | 黎志康. 我国水稻分子育种计划的策略. 分子植物育种, 2005, 3(5): 603-608. |
/
| 〈 |
|
〉 |