Research Papers

A Proteomic Study on a DiseaseResistanceEnhanced Rice Lesion Mimic Mutant

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  • 1College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; 2 State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Biotechnology in Plant Protection, MOA, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China; 3College of Life Science, China Jiliang University, Hangzhou 310018, China;

Received date: 2014-04-16

  Revised date: 2014-06-27

  Online published: 2014-11-10

Abstract

A lesionmimic mutant in rice (Oryza sativa L.), chloroplasticH2O2induced lesion 1 (chl1), has enhanced resistance to rice blast and bacterial blight. To understand the molecular mechanisms underlying this phenotype and its resistance responses, a proteomicsbased approach was used to identify  differentiallyexpressed proteins between chl1 and its wild type. Using twodimensional fluorescence difference gel electrophoresis technology and mass spectrometry, 70 protein spots were successfully identified, of which 46 were upregulated and 24 were downregulated in the mutant. These differentiallyexpressed proteins are involved in diverse biological processes including disease resistance, photosynthesis, oxidationreduction reaction, amino acid/protein metabolism, chaperoning and carbohydrate metabolism. The complex regulatory network in which these proteins are involved may play an important role in regulating the programmed cell death and the resistance reaction in chl1.

Cite this article

HAN Xueying1,2, YANG Yong2, YU Chulang2, ZHANG Wenhao3 , YE Shenghai2, CHEN Bin2, CHENG Chen2,CHENG Ye2, YAN Chengqi2, CHEN Jianping1,2,* . A Proteomic Study on a DiseaseResistanceEnhanced Rice Lesion Mimic Mutant[J]. Chinese Journal OF Rice Science, 2014 , 28(6) : 559 -569 . DOI: 10.3969/j.issn.1001-7216.2014.06. 001

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