Through marker assisted selection (MAS), four isogenic lines were obtained, which contained both genes qSB9TQ (quantitative resistance to sheath blight) and TAC1TQ (tiller angle control gene), either of them, and none of them in each background of two japonica varieties (Zhenjing 88 and Wulingjing 1). The agronomic traits, yield related components and sheath blight (SB) disease severity of different lines were investigated under three different favorable disease conditions, slight, moderate and severe. Results showed that: 1) In the same background, the lines harboring TAC1TQ showed significantly larger tiller angle than that of lines without this gene, but presenting a consistent phenotype on other agronomic traits. 2) The average SB ratings of the lines showed significant difference among different favorable disease conditions; in slight disease conditions, the four lines showed similar SB ratings with no significant difference, however, in moderate disease conditions, the lines contained both genes showed significantly lower SB ratings than that of the control; in severe disease conditions, the lines with both genes and with qSB9TQ alone all showed significantly lower SB ratings than that of the control, while the lines with TAC1TQ alone had lower SB ratings than that of the control, too, which further confirmed the contribution of qSB9TQ to SB resistance; the negative interaction between TAC1TQ and qSB9TQ on SB resistance was found. 3) In the same disease condition, the lines with different combinations of TAC1TQ and qSB9TQ did not show significant difference on yield related components; along with the increasing SB ratings, the plant yield, seed setting rate and 1000grain weight of all lines declined; the yield per plant of the four lines in severe disease conditions were all significantly reduced compared to those in slight disease conditions, and among them the control lines suffered from the biggest yield decline. Although the potential of TAC1TQ in japonica rice breeding needs further estimation, introducing both genes TAC1TQ and qSB9TQ is benefit to enhance rice SB resistance.
CHEN Zongxiang1, ZUO Shimin1,*, ZHANG Yafang1, CHEN Hongqi4, FENG Minghui1, JIANG Wei1, FENG Fan1, MA Yuyin3, HUA Heliang2, LI Guosheng2, PAN Xuebiao1,*
. Breeding Potential and Interaction Effects of qSB9TQ and TAC1TQ in Rice Breeding Against Sheath Blight Disease[J]. Chinese Journal OF Rice Science, 2014
, 28(5)
: 479
-486
.
DOI: 10.3969/j.issn.1001-7216.2014.05.005
\[1\]国广泰史，钱前，佐藤宏之，等． 水稻纹枯病抗性QTL分析． 遗传学报，2002，29（1）： 5055.
\[2\]Li Z K, Pinson S R M, Marshetti M A, et al. Characterization of quantitative trait loci QTLs in cultivated rice contributing to field resistance to sheath blight (Rhizotonia solani). Theor Appl Genet, 1995, 91: 374381.
\[3\]Han Y, Xing Y Z, Gu S L, et al. Effect of morphological traits on sheath blight resistance in rice.Acta Bot Sin, 2003, 45(7)： 825831.
\[4\]李桦， 宋成艳， 丛万彪. 粳稻品种抗纹枯病性鉴定与筛选.植物保护， 2000： 26，1921.
\[5\]陈宗祥， 左世敏， 王龙平， 等. 江苏省“十五”前后主栽粳稻品种纹枯病抗性评价.扬州大学学报， 2010， 31（2）： 3135.
\[6\]刘永峰， 陈志谊， 吉健安， 等. 江苏省水稻主栽及区试品种对水稻纹枯病的抗性分析.江苏农业科学， 2006（1）： 2728.
\[7\]Zuo S M, Zhang L, Wang H, et al. Prospect of the QTLqSB9TQ utilized in molecular breeding program of japonica rice against sheath blight. Acta Genet Sin, 2008, 35(8): 499505.
\[8\]谭彩霞， 纪雪梅， 杨勇， 等. 水稻回交世代中两个抗纹枯病主效数量基因的鉴定与标记辅助选择. 遗传学报， 2005， 24（3）：399405.
\[9\]Yin Y J, Zuo S M, Wang H, et al. Evaluation of the effect of qSB9Tq involved in quantitative resistance to rice sheath blight using nearisogenic lines. Can J Plant Sci, 2009, 89: 731737.
\[10\]陈宗祥， 左示敏， 张亚芳， 等. 水稻抗纹枯病QTL qSB9TQ和抗条纹叶枯病基因Stvbi的聚合育种.作物学报，2012，38（7）： 11781186.
\[11\]王辉. 水稻抗纹枯病QTL qSB9Tq的进一步精细定位. 扬州： 扬州大学，2010.
\[12\]Yu B S, Lin Z W, Li H X, et al. TAC1,a major quantitative trait locus controlling tiller angle in rice. Plant J， 2007, 52: 891898.
\[13\]潘学彪, 陈宗祥, 徐敬友, 等. 不同接种调查方法对抗水稻纹枯病遗传研究的影响. 江苏农学院学报, 1997, 18(3): 2732.
\[14\]左示敏, 张亚芳, 殷跃军, 等. 田间水稻纹枯病抗性鉴定体系的确立与完善. 扬州大学学报: 农业与生命科学版, 2006, 27(4): 5761.
\[15\]陈志谊, 殷尚智, 陆凡, 等. 稻种资源对水稻纹枯病抗性鉴定初报. 植物保护, 1994(6)：2325.
\[16\]陈志谊, 陆凡, 刘永锋, 等. 江苏省水稻主栽及区试品种抗病性鉴定和评价. 江苏农业学报, 2001, 17(2): 8286.
\[17\]潘学彪, Rush M C. 美国的水稻纹枯病抗病遗传育种研究. 江苏农学院学报, 1997, 18(1): 5764.
\[18\]陈宗祥, 邹军煌, 徐敬友, 等. 对水稻纹枯病抗源的初步研究. 中国水稻科学, 2000, 14(1): 1518.
\[19\]张舒, 陈其志, 吕亮, 等. 自然诱发条件下湖北省水稻主栽品种对稻瘟病、纹枯病的抗性鉴定. 华中农业大学学报, 2006, 25: 236240.
\[20\]张楷正, 李平, 李娜, 等. 水稻抗纹枯病种质资源、抗性遗传和育种研究进展分子. 植物育种, 2006, 4(5): 713720.
\[21\]左示敏, 张亚芳, 陈宗祥, 等. 水稻抗纹枯病遗传育种研究进展. 中国科学: C辑, 2010, 40(11): 10141023.
\[22\]陈志谊, 过崇俭, 杨金生. 水稻品种间纹枯病的严重度与产量损失的关系. 植物保护学报, 1989, 16: 4954.
\[23\]Chen Z X, Zhang Y F, Feng F, et al. Improvement of japonica rice resistance to sheath blight by pyramiding qSB9TQ and qSB7TQ. Field Crops Res, 2014, 161: 118127.
\[24\]Zhu Y J, Zuo S M, Chen Z X, et al. Identification of two major QTLs with resistance to rice sheath blight in field tests using chromosome single segment substitution lines. Plant Dis, 2014, 958: 11121121.
