OsLSD2 is a member of LSD1 gene family， which encodes C2C2 zinc finger protein gene. As it is known that LSD1 family is involved in disease resistance. In order to create environmentfriendly rice plant, we investigated the effects of overexpression of this gene in Nipponbare (WT) on plant growth and nitrogen use efficiency. The T3 generation plants showed that the plant height was reduced significantly；tiller number was averagely decreased by two; seed setting rate was only 64%-72%; the grain yield was reduced by 33%-42% compared with WT; the growth duration was shortened by 15 days; and the absorption and utilization rate of nitrogen were reduced significantly as OsLSD2 overexpressed. The phenotype of transformed lines suggested the overexpression of OsLSD2 can significantly repress the growth and nitrogen translocation in rice.
TONG Jun#， CHEN Jingguang#， ZHU Jingwen， FAN Xiaorong*， XU Guohua
. Effects of Rice C2C2 Zinc Finger Protein Gene OsLSD2 on the Growth and Nitrogen Utilization of Nipponbare[J]. Chinese Journal OF Rice Science, 2014
, 28(4)
: 435
-441
.
DOI: 10.3969/j.issn.1001-7216.2014.04.013
\[1\]徐春春, 李凤博, 周锡跃, 等. 提高水稻氮肥利用率及效果研究进展. 浙江农业科学, 2012(1): 98101.
\[2\]薛应征, 刘贺梅, 殷春渊, 等. 氮肥与密度对水稻产量及构成因素影响. 耕作与栽培, 2011(1): 1920.
\[3\]董明辉, 张洪程, 戴其根. 不同粳稻品种氮素吸收利用特点的研究. 扬州大学学报, 2002, 23(4): 4347.
\[4\]江立庚, 曹卫星. 水稻高效利用氮素的生理机制与有效途径. 中国水稻科学, 2002, 16(3): 261264.
\[5\]Bethke P C, Jones R L. Cell death of barley aleurone protoplasts is mediated by reactive oxygen species. Planta, 2001, 25: 1929.
\[6\]Dietrich R A, Delaney T P,Uknes S J, et al. Arabidopsis mutants simulating disease resistance response. Cell, 1994, 77: 565578.
\[7\]Dietrich R A,Richberg M H, Schmidt R, et al. A novel zinc finger protein is encoded by the Arabidopsis LSD1 gene and functions as a negative regulator of plant cell death. Cell, 1997, 88: 685694.
\[8\]He S, Huang K, Zhang X, et al. The LSD1type zinc finger motifs of Pisum sativa LSD1 are a novel nuclear localization signal and interact with importin Alpha. PloS One, 2011, 6(7): 2213122142.
\[9\]Liu Q P, Xue Q Z. Evolution, and functional divergence of the LSD1like gene family: Inference from the rice genome. Mol Phylogeny, 2007, 64: 354363.
\[10\]Xiao Z H, Yan S, Xiao Y Z. The Arabidopsis LSD1 gene plays an important role in the regulation of low temperaturedependent cell death. New Phytol, 2010, 187: 301312.
\[11\]Wang L, Pei Z, Tian Y, et al. OsLSD1, a rice zinc finger protein, regulates programmed cell death and callus differentiation. Mol PlantMicrobe Int, 2005, 18: 375384.
\[12\]王丽娟, 田颖川, 何朝族. 新基因水稻OsLSD1的克隆及拟南芥和水稻类LSD1基因家族的生物信息学分析. 生物化学与生物物理进展, 2005, 32(3): 268274.
\[13\]Kliebenstein D J, Dietrich R A, Martin A C, et al. LSD regulates salicylic acid induction of copper zinc superoxide dismutase in Arabidopsis thaliana. Mol PlantMicrobe Int, 1999, 12(11): 10221026.
\[14\]Kaminaka H, Nakc C, Epple P, et al. bZIP10LSD1 antagonism modulates basal defense and cell death in Arabidopsis following infection. EMBO J, 2006, 25(18): 44004411.
\[15\]Muhlenbock P, Plaszczyca M, Plaszczyca M, et al. Lysigenous aerenchyma formation in Arabidopsis is controlled by LESION SIMULATING DISEASE1. Plant Cell, 2007, 19(11): 38193830.
\[16\]Fan X R, Shen Q R, Ma Z Q, et al. A comparison of nitrate transport in four different rice (Oryza sativa L) cultivars characterizing the NO3－ and NH4＋ uptake process of rice roots by use of N15 labelled NO3－ and NH4＋. Plant Physiol, 2005, 18: 313320.
\[17\]Fan X R, Jia L J, Li Y L, et al. Comparing nitrate storage and remobilization in two rice cultivars that differ in their nitrogen use efficiency. J Exp Bot, 2007, 58(7): 17291740.
\[18\]Rusterucci C, Aviv D H, Holt B F,et al. The disease resistance signaling components EDS1 and PAD4 are essential regulators of the cell death pathway controlled by LSD1 in Arabidiosis. Plant Cell, 2001, 13(10): 22112224.
\[19\]Epple P, Mack A Ａ, Morris V R F, et al. Antagonistic control of oxidative stressinduced cell death in Arabidopsis by two related, plantspecific zinc finger proteins. Proc Natl Acad Sci USA, 2003, 100(11): 68316836.
\[20\]Keisa A, Kanberga K, Gill U, et al. Cloning and characterization of barley homologues of the Arabidopsis LSD1 gene: Putative regulators of hypersensitive response. Acta Univ Latviensis, 2008, 745: 87101.
\[21\]Kliebenstein D J, Dietrich R A, Martin A C, et al. LSD regulates salicylic acid induction of copper zinc superoxide dismutase in Arabidopsis thaliana. Mol PlantMicrobe Int, 1999, 12(11): 10221026.
\[22\]Li Y L, Fan X R, Shen Q R. The relationship between rhizosphere nitrification and nitrogen use efficiency in rice plants. Plant Cell Environ, 2008, 31(1): 7385.
